SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC
文献类型：期刊论文
| 作者 | He Dajun; Huang Yun; Ayupbek Amatjan; Gu Dongyu; Yang Yi; Aisa Haji Akber ; Ito Yoichiro
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| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2010 |
| 卷号 | 33期号:5页码:615-628 |
| 关键词 | black currant high-speed countercurrent chromatography (HSCCC) hyperoside isoquercitrin kaempferol 3-O-galactoside kaempferol 3-O-glucoside |
| ISSN号 | 10826076 |
| 通讯作者 | Aisa, HA |
| 英文摘要 | High-speed countercurrent chromatography (HSCCC) has been successfully used for the preparative isolation of flavonoids from the ethyl acetate extracts of black currant leaves. The HSCCC separation was performed with a two-phase solvent system composed of n-hexane/EtOAc/MeOH/H2O (1:10:1:10, v/v) at a flow rate of 1.5mL/min. When the flow rate was increased from 1.0 to 3.0mL/min, the retention of stationary phase decreased from 60.3% to 39.7% resulting in a loss of peak resolution, while the stationary phase retention is stable with an increase in sample size from 25 to 200mg. From 100mg of the crude sample, HSCCC separation yielded 4.0mg of kaempferol 3-O-galactoside, 6.0mg of kaempferol 3-O-glucoside, and 9.0mg of fraction I containing a mixture of hyperoside and isoquercitrin. Then, from 18mg of fraction I, 3.0mg of hyperoside and 11.0mg isoquercitrin were separated by preparative HPLC by successive sample injection at every 100min interval. Chemical structures of all these compounds were confirmed by MS and NMR. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 收录类别 | SCI |
| WOS记录号 | WOS:000275112200003 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1787]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Shihezi Univ, Sch Life Sci, Shihezi, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing, Peoples R China;NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA |
| 推荐引用方式 GB/T 7714 | He Dajun,Huang Yun,Ayupbek Amatjan,et al. SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2010,33(5):615-628. |
| APA | He Dajun.,Huang Yun.,Ayupbek Amatjan.,Gu Dongyu.,Yang Yi.,...&Ito Yoichiro.(2010).SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,33(5),615-628. |
| MLA | He Dajun,et al."SEPARATION AND PURIFICATION OF FLAVONOIDS FROM BLACK CURRANT LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HPLC".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 33.5(2010):615-628. |
入库方式： OAI收割
来源：新疆理化技术研究所
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