One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography
文献类型：期刊论文
| 作者 | Yang Yi; Gu Dongyu; Yili Abulimiti ; Zhao Yongxin; He Dajun; Aisa Haji Akber; Aisa, HA
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2010 |
| 卷号 | 21期号:2页码:205-209 |
| 关键词 | high-speed counter-current chromatography Artemisia rupestris L. rupestonic acid chrysosptertin B one-step separation |
| ISSN号 | 0958-0344 |
| 通讯作者 | Aisa, HA |
| 英文摘要 | Introduction - Artemisia rupestris L. is a well-known traditional Chinese medicinal plant in Xinjiang. Rupestonic acid is the main active ingredient of A. rupestris L., and has been chosen as a 'marker compound' for the chemical evaluation or quality control of A. rupestris L. and its products. Although HSCCC separation method was developed before, the separation was performed with two steps using the same solvent system, which were time-consuming and waste of the solvents. Objective - To develop a simple HSCCC method for the separation and purification of rupestonic acid in a single run. Methodology - The measurement of partition coefficient (K) was introduced to select the two-phase solvent system. The simple HSCCC method was established according to the selected solvent system for separation and purification of rupestonic acid. The purity of target compound was test by HPLC and the structure was identified by MS,(1)H NMR and (13)C NMR. Results - A total of 72.3 mg of rupestonic acid and 53.5 mg of chrysosptertin B with over 95% purity were yielded from 500 mg extracts of Artemisia rupestris L. in one-step separation. Conclusion - The rupestonic acid was separated in a single run by HSCCC. Copyright (C) 2009 John Wiley & Sons, Ltd. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences ; Chemistry |
| 收录类别 | SCI |
| WOS记录号 | WOS:000275007400011 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1869]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
| 通讯作者 | Aisa, HA |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Yi,Gu Dongyu,Yili Abulimiti,et al. One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography[J]. PHYTOCHEMICAL ANALYSIS,2010,21(2):205-209. |
| APA | Yang Yi.,Gu Dongyu.,Yili Abulimiti.,Zhao Yongxin.,He Dajun.,...&Aisa, HA.(2010).One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography.PHYTOCHEMICAL ANALYSIS,21(2),205-209. |
| MLA | Yang Yi,et al."One-step Separation and Purification of Rupestonic Acid and Chrysosptertin B from Artemisia rupestris L. by High-speed Counter-current Chromatography".PHYTOCHEMICAL ANALYSIS 21.2(2010):205-209. |
入库方式： OAI收割
来源：新疆理化技术研究所
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