Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Yang Yi; Aisa Haji Akber; Ito Yoichiro |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2009 |
| 卷号 | 1216期号:27页码:5265-5271 |
| 关键词 | Spiral tube assembly Flat-twisted tubing High-speed counter-current chromatography Retention of the stationary phase Resolution Separation of DNP-amino acid, dipeptide and protein |
| ISSN号 | 0021-9673 |
| 英文摘要 | The original spiral tube assembly for high-speed counter-current chromatography (HSCCC) is further improved by a new tube configuration called "flat-twisted tubing" which was made by extruding the tube (1.6 mm 1.D.) through a narrow slot followed by twisting along its axis forming about I cm twisted screw pitch. This modification interrupts the laminar How of the mobile phase through the tube and continuously mixes the two phases through the column. The performance of this spiral tube assembly was tested by three types of two-phase solvent systems with different polarities each with a set of suitable test samples such as DNP-amino acids, dipeptides and proteins at the optimal elution modes. In general all these test samples yielded higher resolution with the lower mobile phase than the upper mobile phase. In the most hydrophobic two-phase solvent system composed of hexane-ethyl acetate-methanol-0.1 M hydrochloric acid (1:1:1:1, v/v/v/v), DNP-amino acids were separated with Rs-a (peak resolution based on the same Column capacity adjusted for comparison) at 4.40 and 73% of stationary phase retention at a flow rate of 0.5 ml/min with the lower mobile phase. In the polar solvent system composed of 1 butanol-acetic acid-water (4:1:5, v/v/v), dipeptide samples were resolved with Rs-a at 4.06, compared to 2.79 with the cross-pressed tube assembly at 45% stationary phase retention, each at a flow rate of 1 ml/min. Finally in the aqueous-aqueous polymer phase systems composed of polyethylene glycol 1000 - dibasic potassium phosphate each 12.5% (w/w) in water, protein samples were resolved with Rs-a at 2.53 compared to 1.10 with the cross-pressed tube assembly at 52% of stationary phase retention,each at a now rate of 1 ml/min. These results indicate that the present system substantially improves the partition efficiency with a satisfactory level of stationary phase retention by the lower mobile phase. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| WOS记录号 | WOS:000267559800014 |
| 公开日期 | 2012-11-29 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/1884]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 |
| 作者单位 | NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang Yi,Aisa Haji Akber,Ito Yoichiro. Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2009,1216(27):5265-5271. |
| APA | Yang Yi,Aisa Haji Akber,&Ito Yoichiro.(2009).Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1216(27),5265-5271. |
| MLA | Yang Yi,et al."Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1216.27(2009):5265-5271. |
入库方式： OAI收割
来源：新疆理化技术研究所
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