Pretreatment incubation for culture and cryopreservation of Sabal embryos
文献类型：期刊论文
| 作者 | Wen, B ; Wang, RL |
| 刊名 | PLANT CELL TISSUE AND ORGAN CULTURE
 |
| 出版日期 | 2010 |
| 卷号 | 102期号:2页码:237-243 |
| 关键词 | Cryopreservation Embryo culture Preincubation Sabal Zygotic embryo |
| 中文摘要 | Zygotic embryos from mature seeds of Sabal jamaicensis, S. minor, S. umbraculifera and S. yapa were cultured in vitro and cryopreserved successfully. Seed pretreatment prior to embryo isolation was shown to be crucial. Soaking seeds in water or sowing on 1% agar followed by incubation at 30A degrees C for 1 day (S. jamaicensis, S. minor and S. umbraculifera) or 3 days (S. yapa only) prior to embryo isolation increased the percentage emergence of cultured embryos from less than 20% to more than 94%. Without this pretreatment, most isolated embryos turned brown and died soon after culture. Through preincubation combined with partial dehydration to 18-28% moisture content, direct freezing and rapid thawing, isolated embryos were successfully cryopreserved and 31-44% emergence was achieved from postthawed embryos for the four Sabal species. |
| 公开日期 | 2012-12-26 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/3846]  |
| 专题 | 西双版纳热带植物园_支撑系统 |
| 推荐引用方式 GB/T 7714 | Wen, B,Wang, RL. Pretreatment incubation for culture and cryopreservation of Sabal embryos[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2010,102(2):237-243. |
| APA | Wen, B,&Wang, RL.(2010).Pretreatment incubation for culture and cryopreservation of Sabal embryos.PLANT CELL TISSUE AND ORGAN CULTURE,102(2),237-243. |
| MLA | Wen, B,et al."Pretreatment incubation for culture and cryopreservation of Sabal embryos".PLANT CELL TISSUE AND ORGAN CULTURE 102.2(2010):237-243. |
入库方式： OAI收割
来源：西双版纳热带植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。