The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum
文献类型：期刊论文
| 作者 | Xu, ZF*
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:11页码:15162-15176 |
| 关键词 | PIN2 proteinase inhibitor Solanum americanum insect resistance trichome |
| 中文摘要 | Proteinase inhibitors play an important role in plant resistance of insects and pathogens. In this study, we characterized the serine proteinase inhibitor SaPIN2b, which is constitutively expressed in Solanum americanum trichomes and contains two conserved motifs of the proteinase inhibitor II (PIN2) family. The recombinant SaPIN2b (rSaPIN2b), which was expressed in Escherichia coli, was demonstrated to be a potent proteinase inhibitor against a panel of serine proteinases, including subtilisin A, chymotrypsin and trypsin. Moreover, rSaPIN2b also effectively inhibited the proteinase activities of midgut trypsin-like proteinases that were extracted from the devastating pest Helicoverpa armigera. Furthermore, the overexpression of SaPIN2b in transgenic tobacco plants resulted in enhanced resistance against H. armigera. Taken together, our results demonstrated that SaPIN2b is a potent serine proteinase inhibitor that may act as a protective protein in plant defense against insect attacks. |
| 公开日期 | 2013-01-08 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/3869]  |
| 专题 | 西双版纳热带植物园_能源植物分子育种研究组 |
| 推荐引用方式 GB/T 7714 | Xu, ZF*. The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(11):15162-15176. |
| APA | Xu, ZF*.(2012).The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(11),15162-15176. |
| MLA | Xu, ZF*."The Characterization of SaPIN2b, a Plant Trichome-Localized Proteinase Inhibitor from Solanum americanum".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.11(2012):15162-15176. |
入库方式： OAI收割
来源：西双版纳热带植物园
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