Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae
文献类型：期刊论文
| 作者 | Chen, Lianguo1,2; Huang, Changjiang3; Hu, Chenyan1; Yu, Ke1; Yang, Lihua1; Zhou, Bingsheng1 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 31期号:10页码:2338-2344 |
| 关键词 | Polybrominated diphenyl ethers Developmental neurotoxicity Cholinergic system Locomotor behavior Zebrafish embryos |
| ISSN号 | 0730-7268 |
| 通讯作者 | Zhou, BS (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China. |
| 中文摘要 | The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120?h postfertilization zebrafish embryos were exposed to DE-71 (0, 31.0, 68.7, and 227.6?mu g/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6?mu g/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6?mu g/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (a1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of a1-tubulin was also decreased. Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development. Environ. Toxicol. Chem. 2012; 31: 23382344. (c) 2012 SETAC |
| 英文摘要 | The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120?h postfertilization zebrafish embryos were exposed to DE-71 (0, 31.0, 68.7, and 227.6?mu g/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6?mu g/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6?mu g/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (a1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of a1-tubulin was also decreased. Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development. Environ. Toxicol. Chem. 2012; 31: 23382344. (c) 2012 SETAC |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | POLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME-RETARDANT ; NEONATAL EXPOSURE ; NEURONAL DEVELOPMENT ; EMBRYONIC EXPOSURE ; MATERNAL TRANSFER ; CEREBRAL-CORTEX ; EXPRESSION ; GROWTH ; SYNAPTOGENESIS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] |
| 语种 | 英语 |
| WOS记录号 | WOS:000308928700019 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17250]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Lianguo,Huang, Changjiang,Hu, Chenyan,et al. Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2012,31(10):2338-2344. |
| APA | Chen, Lianguo,Huang, Changjiang,Hu, Chenyan,Yu, Ke,Yang, Lihua,&Zhou, Bingsheng.(2012).Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,31(10),2338-2344. |
| MLA | Chen, Lianguo,et al."Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 31.10(2012):2338-2344. |
入库方式： OAI收割
来源：水生生物研究所
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