A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments
文献类型：期刊论文
| 作者 | Zhou, Quan1,2; Chen, Wei1; Zhang, Huiyong3; Peng, Liang1; Liu, Liming1; Han, Zhiguo3; Wan, Neng4; Li, Lin1; Song, Lirong1 |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2012 |
| 卷号 | 24期号:9页码:1709-1716 |
| 关键词 | flow cytometry benthic Microcystis cell enumeration colony size measurement |
| ISSN号 | 1001-0742 |
| 通讯作者 | Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw (dry weight)/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x10(4) cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 mu m in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystis flos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different-sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium. |
| 英文摘要 | A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw (dry weight)/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x10(4) cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 mu m in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystis flos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different-sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | AERUGINOSA KUTZ ; EUTROPHIC LAKE ; MEILIANG BAY ; PHYTOPLANKTON ; CYANOPHYCEAE ; RECRUITMENT ; SHALLOW ; MARINE ; WATER ; CYTOBUOY |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program (973) of China [2008CB418006]; National Special Program of Water Environment [2009ZX07106-001-002]; National Natural Science Foundation of China [31070355]; National Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07101-013] |
| 语种 | 英语 |
| WOS记录号 | WOS:000309371600022 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17282]  |
| 专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Zealquest Sci Technol Co Ltd, Zealquest Lab Ecol Res, Shanghai 200333, Peoples R China 4.Changshu Inst Technol, Changshu 215500, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Quan,Chen, Wei,Zhang, Huiyong,et al. A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2012,24(9):1709-1716. |
| APA | Zhou, Quan.,Chen, Wei.,Zhang, Huiyong.,Peng, Liang.,Liu, Liming.,...&Song, Lirong.(2012).A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,24(9),1709-1716. |
| MLA | Zhou, Quan,et al."A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 24.9(2012):1709-1716. |
入库方式： OAI收割
来源：水生生物研究所
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