Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus
文献类型：期刊论文
| 作者 | Huang, Rong; Dong, Feng2; Jang, Songhun2; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 38期号:2页码:383-388 |
| 关键词 | Grass carp BAC library tlr4 gene cluster Grass carp reovirus |
| ISSN号 | 0145-305X |
| 通讯作者 | Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China. |
| 中文摘要 | The mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | RESPIRATORY SYNCYTIAL VIRUS ; INNATE IMMUNITY ; ACTIVATION ; RECOGNITION ; EXPRESSION ; PREDICTION ; EVOLUTION ; FAMILY ; FISH ; LPS |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] |
| 语种 | 英语 |
| WOS记录号 | WOS:000309312700021 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17226]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Rong,Dong, Feng,Jang, Songhun,et al. Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(2):383-388. |
| APA | Huang, Rong,Dong, Feng,Jang, Songhun,Liao, Lanjie,Zhu, Zuoyan,&Wang, Yaping.(2012).Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(2),383-388. |
| MLA | Huang, Rong,et al."Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.2(2012):383-388. |
入库方式： OAI收割
来源：水生生物研究所
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