Identification, characterization and the interaction of Tollip and IRAK-1 in grass carp (Ctenopharyngodon idellus)
文献类型：期刊论文
| 作者 | Huang, Rong1; Lv, Jianjian1,2; Luo, Daji1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 33期号:3页码:459-467 |
| 关键词 | Tollip IRAK-1 Molecular cloning Viral infection Gras g carp |
| ISSN号 | 1050-4648 |
| 通讯作者 | Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China. |
| 中文摘要 | Tollip and IRAK-1 are key components of the TLR/IL-1R signaling pathway in mammals, which play crucial roles as mediators of the TLR/IL-1R signal transduction pathways. Although several TLRs have been found in fish, molecular associations, protein protein interactions or the role of the TLR signaling pathway in infection-induced immunity in fish has received little attention. In this study. Tollip and IRAK-1 sequences of grass carp were isolated from a head kidney cDNA library. Full length transcripts and sequences of promoter regions were obtained by 3' and 5' RACE and genome walking, respectively. Reporter gene-promoter constructs and real-time RT-PCR analysis was used to determine grass carp Tollip and IRAK-1 transcription pattern in tissues. Recombinant proteins were used for antibodies production. Phylogenetically, the grass carp loci clustered with previously reported Tollip and IRAK-1genes, respectively, and their sequences shared the highest identity with the genes of zebrafish (Danio rerio). The promoter region of grass carp Tollip and IRAK-1 proved to be active. After viral infection transcript levels of both loci were upregulated in most immune-related tissues in a time-dependent manner. Using antibodies produced in this study, immunofluorescence analysis indicated that Tollip and IRAK-1 were uniformly distributed and co-localized in the cytoplasm of CIK cells. After viral infection, however, Tollip and IRAK-1 both trended toward the cell membrane. Our results demonstrate the existence of Tollip and IRAK-1 proteins in teleost species, and suggest that Tollip-IRAK-1 complexes are being recruited to receptor complexes after stimulation with virus. These results provide novel insights into the role of the TLR signaling pathway in teleosts, especially the action of teleost Tollip and IRAK-1 and the interaction of these molecules as part of this pathway. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Tollip and IRAK-1 are key components of the TLR/IL-1R signaling pathway in mammals, which play crucial roles as mediators of the TLR/IL-1R signal transduction pathways. Although several TLRs have been found in fish, molecular associations, protein protein interactions or the role of the TLR signaling pathway in infection-induced immunity in fish has received little attention. In this study. Tollip and IRAK-1 sequences of grass carp were isolated from a head kidney cDNA library. Full length transcripts and sequences of promoter regions were obtained by 3' and 5' RACE and genome walking, respectively. Reporter gene-promoter constructs and real-time RT-PCR analysis was used to determine grass carp Tollip and IRAK-1 transcription pattern in tissues. Recombinant proteins were used for antibodies production. Phylogenetically, the grass carp loci clustered with previously reported Tollip and IRAK-1genes, respectively, and their sequences shared the highest identity with the genes of zebrafish (Danio rerio). The promoter region of grass carp Tollip and IRAK-1 proved to be active. After viral infection transcript levels of both loci were upregulated in most immune-related tissues in a time-dependent manner. Using antibodies produced in this study, immunofluorescence analysis indicated that Tollip and IRAK-1 were uniformly distributed and co-localized in the cytoplasm of CIK cells. After viral infection, however, Tollip and IRAK-1 both trended toward the cell membrane. Our results demonstrate the existence of Tollip and IRAK-1 proteins in teleost species, and suggest that Tollip-IRAK-1 complexes are being recruited to receptor complexes after stimulation with virus. These results provide novel insights into the role of the TLR signaling pathway in teleosts, especially the action of teleost Tollip and IRAK-1 and the interaction of these molecules as part of this pathway. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | RECEPTOR-ASSOCIATED KINASE ; INTERLEUKIN-1 RECEPTOR ; IL-1 RECEPTOR ; PARALICHTHYS-OLIVACEUS ; SIGNALING PATHWAYS ; NEGATIVE REGULATOR ; MOLECULAR-CLONING ; JAPANESE FLOUNDER ; GENE-EXPRESSION ; INNATE IMMUNITY |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [31130055]; National Basic Research Program of China [2009CB118701] |
| 语种 | 英语 |
| WOS记录号 | WOS:000308057700001 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17228]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Rong,Lv, Jianjian,Luo, Daji,et al. Identification, characterization and the interaction of Tollip and IRAK-1 in grass carp (Ctenopharyngodon idellus)[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):459-467. |
| APA | Huang, Rong,Lv, Jianjian,Luo, Daji,Liao, Lanjie,Zhu, Zuoyan,&Wang, Yaping.(2012).Identification, characterization and the interaction of Tollip and IRAK-1 in grass carp (Ctenopharyngodon idellus).FISH & SHELLFISH IMMUNOLOGY,33(3),459-467. |
| MLA | Huang, Rong,et al."Identification, characterization and the interaction of Tollip and IRAK-1 in grass carp (Ctenopharyngodon idellus)".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):459-467. |
入库方式： OAI收割
来源：水生生物研究所
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