Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish Siniperca chuatsi
文献类型：期刊论文
| 作者 | Wang, Gailing1,2,3; Li, Junhua1,2; Zou, Pengfei1,2; Xie, Haixia1; Huang, Bei1,2; Nie, Pin1; Chang, Mingxian1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 33期号:3页码:522-531 |
| 关键词 | Mandarin fish beta-Defensin Gene expression Promoter activity Bactericidal activity |
| ISSN号 | 1050-4648 |
| 通讯作者 | Chang, MX (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | beta-Defensin (BD) are cysteine-rich, cationic antimicrobial peptides which play an important role in innate immune system against invading microbes. In the present study, the cDNA cloning, expression analysis, transcriptional regulation and antimicrobial activity of beta-defensin (ScBD) from mandarin fish (Siniperca chuatsi) were characterized. The cDNA sequence of ScBD is 596 bp which encodes a protein of 63 amino acids (aa). The ScBD gene comprises three exons and two introns. The signal peptide is located in the first exon. ScBD contains 6 cysteines, and belongs to fish defensin 2 group based on phylogenetic analysis. Real-time quantitative PCR results showed that the mRNA transcripts of ScBD were distributed mainly in mucosal and lymphoid organs/tissues including intestine, gill, head kidney, kidney and spleen, with the highest level observed in spleen. Western blotting analysis revealed that the ScBD protein was abundant in head kidney, gill and spleen. A total of 3268 bp 5' flanking region of the ScBD gene promoter was sequenced, which contained a number of putative transcriptional binding sites for transcription factors. These transcription factors were analyzed using in vitro luciferase assay. The DNA region from position of -705 to -498 bp contains positive regulatory elements and that of -227 to +54 bp harbors the TATA which is essential for initiating gene expression. In addition, the ScBD peptide showed antibacterial activity against Escherichia call M15, Staphylococcus aureus and Aeromonas hydrophila, whilst no effect on Edwardsiella tarda. These data suggest that the ScBD is importantly involved in host immune responses to invasion of bacterial pathogens. (C) 2012 Elsevier Ltd. All rights reserved. |
| 英文摘要 | beta-Defensin (BD) are cysteine-rich, cationic antimicrobial peptides which play an important role in innate immune system against invading microbes. In the present study, the cDNA cloning, expression analysis, transcriptional regulation and antimicrobial activity of beta-defensin (ScBD) from mandarin fish (Siniperca chuatsi) were characterized. The cDNA sequence of ScBD is 596 bp which encodes a protein of 63 amino acids (aa). The ScBD gene comprises three exons and two introns. The signal peptide is located in the first exon. ScBD contains 6 cysteines, and belongs to fish defensin 2 group based on phylogenetic analysis. Real-time quantitative PCR results showed that the mRNA transcripts of ScBD were distributed mainly in mucosal and lymphoid organs/tissues including intestine, gill, head kidney, kidney and spleen, with the highest level observed in spleen. Western blotting analysis revealed that the ScBD protein was abundant in head kidney, gill and spleen. A total of 3268 bp 5' flanking region of the ScBD gene promoter was sequenced, which contained a number of putative transcriptional binding sites for transcription factors. These transcription factors were analyzed using in vitro luciferase assay. The DNA region from position of -705 to -498 bp contains positive regulatory elements and that of -227 to +54 bp harbors the TATA which is essential for initiating gene expression. In addition, the ScBD peptide showed antibacterial activity against Escherichia call M15, Staphylococcus aureus and Aeromonas hydrophila, whilst no effect on Edwardsiella tarda. These data suggest that the ScBD is importantly involved in host immune responses to invasion of bacterial pathogens. (C) 2012 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; NF-KAPPA-B ; ANTIMICROBIAL PEPTIDES ; FUNCTIONAL-CHARACTERIZATION ; ADAPTIVE IMMUNITY ; DENDRITIC CELLS ; HELA-CELLS ; INNATE ; GENE ; BETA-DEFENSIN-2 |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [U0631010]; Government of Guangdong Province; National Basic Research Program of China (973 Program) [2009CB118703] |
| 语种 | 英语 |
| WOS记录号 | WOS:000308057700007 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17230]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Huanghuai Univ, Dept Bioengn, Zhumadian 463000, Henan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Gailing,Li, Junhua,Zou, Pengfei,et al. Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish Siniperca chuatsi[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(3):522-531. |
| APA | Wang, Gailing.,Li, Junhua.,Zou, Pengfei.,Xie, Haixia.,Huang, Bei.,...&Chang, Mingxian.(2012).Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish Siniperca chuatsi.FISH & SHELLFISH IMMUNOLOGY,33(3),522-531. |
| MLA | Wang, Gailing,et al."Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish Siniperca chuatsi".FISH & SHELLFISH IMMUNOLOGY 33.3(2012):522-531. |
入库方式： OAI收割
来源：水生生物研究所
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