Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns
文献类型：期刊论文
| 作者 | Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2012-08-13 |
| 卷号 | 7期号:8页码:e43033 |
| 关键词 | MAJOR CAPSID PROTEIN FISH CELL-LINE FAMILY IRIDOVIRIDAE SEQUENCE-ANALYSIS GENE EXPRESSION DNA MORPHOGENESIS REPLICATION TRANSPORT |
| ISSN号 | 1932-6203 |
| 通讯作者 | Lei, XY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China. |
| 中文摘要 | Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. Methodology/Principal Findings: We cloned and characterized RGV50L, and revealed 50L functions in virus assembly and gene regulation. 50L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix-extension-helix motif. Drug inhibition assay demonstrated that 50L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50L appeared early and persisted in RGV-infected cells following two distribution patterns. One pattern was that 50L exhibited a cytoplasm-nucleus-viromatrix distribution pattern, and mutagenesis of the NLS motif revealed that localization of 50L in the nucleus was NLS-dependent; the other was that 50L co-localized with viral matrix which plays important roles in virus assembly and the life circle of viruses. Conclusion/Significance: RGV 50L is a novel iridovirus IE gene encoded structural protein which plays important roles in virus assembly. |
| 英文摘要 | Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. |
| WOS标题词 | Science & Technology |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | MAJOR CAPSID PROTEIN ; FISH CELL-LINE ; FAMILY IRIDOVIRIDAE ; SEQUENCE-ANALYSIS ; GENE ; EXPRESSION ; DNA ; MORPHOGENESIS ; REPLICATION ; TRANSPORT |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000307500100055 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17232]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Xiao-Ying,Ou, Tong,Zhang, Qi-Ya. Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns[J]. PLOS ONE,2012,7(8):e43033. |
| APA | Lei, Xiao-Ying,Ou, Tong,&Zhang, Qi-Ya.(2012).Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns.PLOS ONE,7(8),e43033. |
| MLA | Lei, Xiao-Ying,et al."Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns".PLOS ONE 7.8(2012):e43033. |
入库方式： OAI收割
来源：水生生物研究所
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