Sequencing and analysis of the complete genome of Rana grylio virus (RGV)
文献类型：期刊论文
| 作者 | Lei, Xiao-Ying; Ou, Tong; Zhu, Ruo-Lin; Zhang, Qi-Ya |
| 刊名 | ARCHIVES OF VIROLOGY
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 157期号:8页码:1559-1564 |
| 关键词 | LYMPHOCYSTIS DISEASE VIRUS RANAVIRUS FAMILY IRIDOVIRIDAE DNA-SEQUENCE FISH CELLS MICRORNAS GENE IDENTIFICATION EXPRESSION APOPTOSIS REPEATS |
| ISSN号 | 0304-8608 |
| 通讯作者 | Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses. |
| 英文摘要 | Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | LYMPHOCYSTIS DISEASE VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; DNA-SEQUENCE ; FISH CELLS ; MICRORNAS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; REPEATS |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] |
| 语种 | 英语 |
| WOS记录号 | WOS:000307253800014 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17234]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Xiao-Ying,Ou, Tong,Zhu, Ruo-Lin,et al. Sequencing and analysis of the complete genome of Rana grylio virus (RGV)[J]. ARCHIVES OF VIROLOGY,2012,157(8):1559-1564. |
| APA | Lei, Xiao-Ying,Ou, Tong,Zhu, Ruo-Lin,&Zhang, Qi-Ya.(2012).Sequencing and analysis of the complete genome of Rana grylio virus (RGV).ARCHIVES OF VIROLOGY,157(8),1559-1564. |
| MLA | Lei, Xiao-Ying,et al."Sequencing and analysis of the complete genome of Rana grylio virus (RGV)".ARCHIVES OF VIROLOGY 157.8(2012):1559-1564. |
入库方式： OAI收割
来源：水生生物研究所
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