Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)
文献类型：期刊论文
| 作者 | Liu, Lusha1,2; Yu, Xiaomu1; Tong, Jingou1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2012-09-01 |
| 卷号 | 39期号:9页码:9211-9221 |
| 关键词 | MSTN Characterization SNP Polymorphism Genetic association Bighead carp (Aristichthys nobilis) |
| ISSN号 | 0301-4851 |
| 通讯作者 | Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | Myostatin (MSTN) is a member of the transforming growth factor-beta superfamily and functions as a negative regulator of skeletal muscle development and growth. In this study, the bighead carp MSTN gene (AnMSTN for short) was cloned and characterized. The 3,769 bp genomic sequence of AnMSTN consisted of three exons and two introns, and the full length cDNA (2,141 bp) of the gene had an open reading frame encoding a polypeptide of 375 amino acids. The deduced amino acid sequence of AnMSTN showed 67.1-98.7 % homology with MSTNs of avian, mammalian and teleostean species. Sequence comparison and phylogenetic analysis confirmed the MSTNs were conserved throughout the vertebrates and AnMSTN belonged to MSNT-1 isoform. AnMSTN was expressed in various tissues with the highest expression in muscle. Two single nucleotide polymorphisms, g.1668T > C in intron 2 and g.2770C > A in 3' UTR, were identified in AnMSTN by sequencing PCR fragments, and genotyped by SSCP. Association analysis showed that g.2770C > A genotypes were significantly associated with total length, body length and body weight (P < 0.01). These results suggest that AnMSTN involves in the regulation of growth, and this polymorphism would be informative for further studies on selective breeding in bighead carp. |
| 英文摘要 | Myostatin (MSTN) is a member of the transforming growth factor-beta superfamily and functions as a negative regulator of skeletal muscle development and growth. In this study, the bighead carp MSTN gene (AnMSTN for short) was cloned and characterized. The 3,769 bp genomic sequence of AnMSTN consisted of three exons and two introns, and the full length cDNA (2,141 bp) of the gene had an open reading frame encoding a polypeptide of 375 amino acids. The deduced amino acid sequence of AnMSTN showed 67.1-98.7 % homology with MSTNs of avian, mammalian and teleostean species. Sequence comparison and phylogenetic analysis confirmed the MSTNs were conserved throughout the vertebrates and AnMSTN belonged to MSNT-1 isoform. AnMSTN was expressed in various tissues with the highest expression in muscle. Two single nucleotide polymorphisms, g.1668T > C in intron 2 and g.2770C > A in 3' UTR, were identified in AnMSTN by sequencing PCR fragments, and genotyped by SSCP. Association analysis showed that g.2770C > A genotypes were significantly associated with total length, body length and body weight (P < 0.01). These results suggest that AnMSTN involves in the regulation of growth, and this polymorphism would be informative for further studies on selective breeding in bighead carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | VERTEBRATE GENOME EVOLUTION ; BETA SUPERFAMILY MEMBER ; DIFFERENTIAL EXPRESSION ; TISSUE EXPRESSION ; ZEBRAFISH ; MUSCLE ; POLYMORPHISMS ; CLONING ; CATTLE ; SHEEP |
| 收录类别 | SCI |
| 资助信息 | Ministry of Agriculture of China [200903045, 2011-G12]; CAS |
| 语种 | 英语 |
| WOS记录号 | WOS:000306799700075 |
| 公开日期 | 2013-01-09 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/17236]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lusha,Yu, Xiaomu,Tong, Jingou. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)[J]. MOLECULAR BIOLOGY REPORTS,2012,39(9):9211-9221. |
| APA | Liu, Lusha,Yu, Xiaomu,&Tong, Jingou.(2012).Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis).MOLECULAR BIOLOGY REPORTS,39(9),9211-9221. |
| MLA | Liu, Lusha,et al."Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)".MOLECULAR BIOLOGY REPORTS 39.9(2012):9211-9221. |
入库方式： OAI收割
来源：水生生物研究所
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