Investigation of the interaction between a bivalent aptamer and thrombin by AFM
文献类型：期刊论文
| 作者 | Ge L; Jin G(靳刚) ; Fang XH; Jin, G
|
| 刊名 | LANGMUIR
 |
| 出版日期 | 2012 |
| 卷号 | 28期号:1页码:707-713 |
| 通讯作者邮箱 | gajin@imech.ac.cn;xfang@iccas.ac.cn |
| 关键词 | Dynamic Force Spectroscopy Single-Molecule Energy Landscape Sinorhizobium-Meliloti Membrane-Proteins Receptor-Binding DNA-Molecules RNA Ligands Inhibitor Recognition |
| ISSN号 | 0743-7463 |
| 产权排序 | [Ge, Lin;Jin, Gang] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China; [Ge, Lin] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; [Jin, Gang] Chinese Acad Sci, Inst Mech, Beijing 100190, Peoples R China; [Fang, Xiaohong] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 通讯作者 | Jin, G ; Chinese Acad Sci, Inst Biophys, 15 Datun Rd, Beijing 100101, Peoples R China. |
| 合作状况 | 国内 |
| 中文摘要 | Aptamers are a new class of molecular probes for protein recognition, detection, and inhibition. Multivalent aptamer protein binding through aptamer assembly has been currently developed as an effective way to achieve higher protein affinity and selectivity. In this study, the specific interaction between bivalent aptamer Bi-8S and thrombin has been measured directly and quantitatively by atomic force microscopy to investigate the unbinding dynamics and dissociation energy landscape of the multivalent interaction. Bivalent aptamer Bi-8S contains thrombin's two aptamers, 15apt and 27apt, which are linked by eight spacer phosphoramidites. The results revealed the sequential dissociation of the two aptamers. Moreover, the dynamic force spectroscopy data revealed that the 27apt's binding to the thrombin remains largely unaffected by the eight-spacer phosphoramidites within Bi-8S. In contrast, the eight-spacer phosphoramidites stabilized the 15apt-thrombin binding. |
| 学科主题 | 生物力学 |
| 分类号 | 一类 |
| 收录类别 | SCI ; EI |
| 资助信息 | We thank Le Mu for experimental advice and Mehdi Damaghi and Prof. Daniel Muller for helpful discussions. This work was supported by the Chinese Academy of Sciences and the National Basic Research Program of China (2011CB911001) and the NSFC (no. 20821003). |
| 原文出处 | http://dx.doi.org/10.1021/la203954x |
| 语种 | 英语 |
| WOS记录号 | WOS:000298904900089 |
| 公开日期 | 2013-01-18 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/46555]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 通讯作者 | Jin, G |
| 推荐引用方式 GB/T 7714 | Ge L,Jin G,Fang XH,et al. Investigation of the interaction between a bivalent aptamer and thrombin by AFM[J]. LANGMUIR,2012,28(1):707-713. |
| APA | Ge L,靳刚,Fang XH,&Jin, G.(2012).Investigation of the interaction between a bivalent aptamer and thrombin by AFM.LANGMUIR,28(1),707-713. |
| MLA | Ge L,et al."Investigation of the interaction between a bivalent aptamer and thrombin by AFM".LANGMUIR 28.1(2012):707-713. |
入库方式： OAI收割
来源：力学研究所
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