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Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior

文献类型：期刊论文

作者Chen, Haitao5,6,7; Zhang, Sheng-Da1,4,5; Chen, Linjie5,6,7; Cai, Yao3; Zhang, Wei-Jia4,5; Song, Tao5,6,7; Wu, Long-Fei2,5
刊名FRONTIERS IN MICROBIOLOGY
出版日期2018-07-17
卷号9页码:13
ISSN号1664-302X
关键词CRISPR-Cas9 magnetotactic bacteria magnetotaxis Amb0994 dynamics simulation
DOI10.3389/fmicb.2018.01569
英文摘要Magnetotactic bacteria (MTB) are a diverse group of microorganisms capable of using geomagnetic fields for navigation. This magnetotactic behavior can help microorganisms move toward favorable habitats for optimal growth and reproduction. A comprehensive understanding of the magnetotactic mechanism at molecular levels requires highly efficient genomic editing tools, which remain underdeveloped in MTB. Here, we adapted an engineered CRISPR-Cas9 system for efficient inactivation of genes in a widely used MTB model strain, Magnetospirillum magneticum AMB-1. By combining a nuclease-deficient Cas9 (dCas9) and single-guide RNA (sgRNA), a CRISPR interference system was successfully developed to repress amb0994 expression. Furthermore, we constructed an in-frame deletion mutant of amb0994 by developing a CRISPR-Cas9 system. This mutant produces normal magnetosomes; however, its response to abrupt magnetic field reversals is faster than wild-type strain. This behavioral difference is probably a consequence of altered flagella function, as suggested with our dynamics simulation study by modeling M. magneticum AMB-1 cell as an ellipsoid. These data indicate that, Amb0994 is involved in the cellular response to magnetic torque changes via controlling flagella. In summary, this study, besides contributing to a better understanding of magnetotaxis mechanism, demonstrated the CRISPR-(d)Cas9 system as a useful genetic tool for efficient genome editing in MTB.
WOS关键词SEQUENCE-SPECIFIC CONTROL ; SP STRAIN AMB-1 ; MAGNETOSOME FORMATION ; GENE-EXPRESSION ; BACTERIAL CHEMORECEPTORS ; CRISPR/CAS9 SYSTEM ; GEOMAGNETIC-FIELDS ; CAS SYSTEM ; CELL ; MAMK
资助项目National Youth Foundation of China[31300689] ; National Key Research and Development Program of China[2017YFC0108501] ; Institute of Electrical Engineering, Chinese Academy of Sciences[Y650141CSA]
WOS研究方向Microbiology
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:000438923900001
资助机构National Youth Foundation of China ; National Youth Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; National Youth Foundation of China ; National Youth Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; National Youth Foundation of China ; National Youth Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences ; National Youth Foundation of China ; National Youth Foundation of China ; National Key Research and Development Program of China ; National Key Research and Development Program of China ; Institute of Electrical Engineering, Chinese Academy of Sciences ; Institute of Electrical Engineering, Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/94018]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Song, Tao; Wu, Long-Fei
作者单位1.Tech Univ Denmark, Dept Biotechnol & Biomed, Lyngby, Denmark
2.Aix Marseille Univ, LCB, CNRS, Marseille, France
3.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing, Peoples R China
4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Deep Sea Microbial Cell Biol, Sanya, Peoples R China
5.Chinese Acad Sci, CNRS Marseille, Rance China Int Lab Evolut & Dev Magnetotact Mult, Beijing, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
7.Chinese Acad Sci, Inst Elect Engn, Beijing Key Lab Biol Electromagnetism, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Haitao,Zhang, Sheng-Da,Chen, Linjie,et al. Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior[J]. FRONTIERS IN MICROBIOLOGY,2018,9:13.
APA Chen, Haitao.,Zhang, Sheng-Da.,Chen, Linjie.,Cai, Yao.,Zhang, Wei-Jia.,...&Wu, Long-Fei.(2018).Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior.FRONTIERS IN MICROBIOLOGY,9,13.
MLA Chen, Haitao,et al."Efficient Genome Editing of Magnetospirillum magneticum AMB-1 by CRISPR-Case9 System for Analyzing Magnetotactic Behavior".FRONTIERS IN MICROBIOLOGY 9(2018):13.

入库方式： OAI收割

来源：地质与地球物理研究所

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