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Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry

文献类型：期刊论文

作者Zuo, Mei-Qing2,3,4; Sun, Rui-Xiang3,4; Fang, Run-Qian1,5; He, Si-Min5; Dong, Meng-Qiu3,4
刊名INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
出版日期2019-11-01
卷号445页码:8
ISSN号1387-3806
关键词Negative-ion mass spectrometry Peptide fragmentation Collision-induced dissociation (CID) Higher-energy collisional dissociation (HCD) Fragmentation efficiency
DOI10.1016/j.ijms.2019.116186
英文摘要Mass spectrometry (MS) analysis of peptides has traditionally been conducted in the positive ion mode on protonated peptides, although negative ion-mode analysis of deprotonated peptides can provide complementary and sometimes critical structural information. This is partly due to insufficient understanding of the fragmentation behaviors of deprotonated peptides. Here, using ion-trap collision-induced dissociation (CID) and higher-energy collisional dissociation (HCD, a beam-type CID), we characterized the fragmentation patterns of 36 deprotonated peptides of 4-16 amino acids on a high-resolution, high-mass accuracy instrument. Our study finds that among the backbone cleavage products, y-, c- and z-type ions (using a nomenclature similar to that of positive ions) are the most dominant species in both CID and HCD spectra of deprotonated peptides, accompanied by abundant neutral loss (NL) peaks. Similar to the charge-dependency of collisional energy of protonated peptides, we find that singly charged deprotonated peptides require significantly higher collisional energy than their doubly or multiply charged counterparts to reach 50% fragmentation. HCD is generally better than CID for peptide sequencing in the negative ion mode, since HCD generates more backbone cleavage products whereas CID produces predominately NL peaks of precursors. For disulfide-bonded peptides and C-terminally amidated peptides, unusual fragmentation is observed in the negative ion mode. The fragmentation behaviors of deprotonated peptides reported in this study will promote further investigation of the fundamental mechanisms and facilitate algorithm development for peptide sequencing in the negative mode. (C) 2019 The Authors. Published by Elsevier B.V.
资助项目National Natural Science Foundation of China ; Ministry of Science and Technology of China ; Beijing Municipal Science and Technology Commission
WOS研究方向Physics ; Spectroscopy
语种英语
出版者ELSEVIER
WOS记录号WOS:000489735800001
源URL[http://119.78.100.204/handle/2XEOYT63/4643]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Sun, Rui-Xiang; Dong, Meng-Qiu
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.China Agr Univ, Coll Biol Sci, Beijing, Peoples R China
3.Natl Inst Biol Sci, Beijing, Peoples R China
4.Tsinghua Univ, Tsinghua Inst Multidisciplinary Biomed Res, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,et al. Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,2019,445:8.
APA Zuo, Mei-Qing,Sun, Rui-Xiang,Fang, Run-Qian,He, Si-Min,&Dong, Meng-Qiu.(2019).Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry.INTERNATIONAL JOURNAL OF MASS SPECTROMETRY,445,8.
MLA Zuo, Mei-Qing,et al."Characterization of collision-induced dissociation of deprotonated peptides of 4-16 amino acids using high-resolution mass spectrometry".INTERNATIONAL JOURNAL OF MASS SPECTROMETRY 445(2019):8.

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来源：计算技术研究所

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