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SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling

文献类型：期刊论文

作者Zhang, Xiaojing1,8; Peng, Yin7,8; Huang, Yong8; Yang, Mengting8; Yan, Ruibin6; Zhao, Yanqiu6; Cheng, Yulan5; Liu, Xi5; Deng, Shiqi8; Feng, Xianling8
刊名CANCER MEDICINE
出版日期2018
卷号7期号:1页码:146-156
ISSN号2045-7634
关键词EMT gastric cancer miR-192/-215 SMG-1 Wnt signaling pathway
DOI10.1002/cam4.1237
英文摘要SMG-1,a member of the phosphoinositide kinase-like kinase family, functioned as a tumor suppressor gene. However, the role of SMG-1 in GC remain uncharacterized. In this study, regulation of SMG-1 by miR-192 and-215, along with the biological effects of this modulation, were studied in GC. We used gene microarrays to screening and luciferase reporter assays were to verify the potential targets of miR-192 and-215. Tissue microarrays analyses were applied to measure the levels of SMG-1 in GC tissues. Western blot assays were used to assess the signaling pathway of SMG-1 regulated by miR-192 and-215 in GC. SMG-1 was significantly downregulated in GC tissues.The proliferative and invasive properties of GC cells were decreased by inhibition of miR-192 and-215, whereas an SMG-1siRNA rescued the inhibitory effects. Finally, SMG-1 inhibition by miR-192 and-215 primed Wnt signaling and induced EMT. Wnt signaling pathway proteins were decreased markedly by inhibitors of miR-192 and-215, while SMG-1 siRNA reversed the inhibition apparently. Meanwhile, miR-192 and-215 inhitibtors increased E-cadherin expression and decreased N-cadherin and cotransfection of SMG-1 siRNA reversed these effects. In summary, these findings illustrate that SMG-1 is suppressed by miR-192 and-215 and functions as a tumor suppressor in GC by inactivating Wnt signaling and suppressing EMT.
资助项目National Nature Science Foundation of China[81772592] ; National Nature Science Foundation of China[31601028] ; National Natural Youth Science Foundation of China[81302151] ; Shenzhen Peacock Plan[KQCX20130621101141669] ; Planned Science and Technology Project of Shenzhen[JCYJ20140418095735574] ; Planned Science and Technology Project of Shenzhen[JCYJ20160422170722474] ; Key Laboratory Project of Shenzhen[ZDSY20130329101130496] ; Medical Science and Technology Research Foundation of Guangdong Province[A2016112] ; Science and Technology Bureau of Shenzhen City[JCYJ20150525092940973] ; NIH[DK087454] ; NIH[CA146799] ; NIH[CA173390] ; American Cancer Society Clinical Research Professorship
WOS研究方向Oncology
语种英语
出版者WILEY
WOS记录号WOS:000425822300015
源URL[http://119.78.100.204/handle/2XEOYT63/5640]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Li, Song; Jin, Zhe
作者单位1.Shenzhen Univ, Sch Med, Guangdong Key Lab Genome Stabil & Dis Prevent, Shenzhen Key Lab Micromol Innovatal Drugs,Shenzhe, Shenzhen, Guangdong, Peoples R China
2.Guangdong Prov Key Lab Mol Oncol Pathol, Guangzhou, Guangdong, Peoples R China
3.Shenzhen Inst Adv Technol, Ctr High Performance Comp, Shenzhen, Guangdong, Peoples R China
4.Guangzhou Med Univ, Dept Pathol & Pathophysiol, Guangzhou, Guangdong, Peoples R China
5.Johns Hopkins Univ, GI Div, Dept Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD USA
6.Peking Univ, Lab Chem Genom, Shenzhen Grad Sch, Shenzhen, Guangdong, Peoples R China
7.Wuhan Univ, Sch Basic Med Sci, Dept Pathol, Wuhan, Hubei, Peoples R China
8.Shenzhen Univ, Sch Med, Dept Pathol, 3688 Nanhai Ave,Rm 703, Shenzhen 518060, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xiaojing,Peng, Yin,Huang, Yong,et al. SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling[J]. CANCER MEDICINE,2018,7(1):146-156.
APA Zhang, Xiaojing.,Peng, Yin.,Huang, Yong.,Yang, Mengting.,Yan, Ruibin.,...&Jin, Zhe.(2018).SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling.CANCER MEDICINE,7(1),146-156.
MLA Zhang, Xiaojing,et al."SMG-1 inhibition by miR-192/-215 causes epithelial-mesenchymal transition in gastric carcinogenesis via activation of Wnt signaling".CANCER MEDICINE 7.1(2018):146-156.

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来源：计算技术研究所

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