Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states
文献类型：期刊论文
| 作者 | Tan, Dan2,3; Li, Qiang1,2,6,7; Zhang, Mei-Jun2; Liu, Chao5; Ma, Chengying4; Zhang, Pan2,3; Ding, Yue-He2,3; Fan, Sheng-Bo5; Tao, Li2,3; Yang, Bing2 |
| 刊名 | ELIFE
 |
| 出版日期 | 2016-03-08 |
| 卷号 | 5页码:51 |
| ISSN号 | 2050-084X |
| DOI | 10.7554/eLife.12509 |
| 英文摘要 | To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing a biotin tag for affinity purification, a chemical cleavage site to separate cross-linked peptides away from biotin after enrichment, and a spacer arm that can be labeled with stable isotopes for quantitation. By locating the flexible proteins on the surface of 70S ribosome, we show that this trifunctional cross-linker is effective at attaining structural information not easily attainable by crystallography and electron microscopy. From a crude Rrp46 immunoprecipitate, it helped identify two direct binding partners of Rrp46 and 15 protein-protein interactions (PPIs) among the co-immunoprecipitated exosome subunits. Applying it to E. coli and C. elegans lysates, we identified 3130 and 893 inter-linked lysine pairs, representing 677 and 121 PPIs. Using a quantitative CXMS workflow we demonstrate that it can reveal changes in the reactivity of lysine residues due to protein-nucleic acid interaction. |
| 资助项目 | National Natural Science Foundation of China ; Ministry of Science and Technology of the People's Republic of China ; Chinese Academy of Sciences |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000373894300001 |
| 出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/8460]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Dong, Meng-Qiu; Lei, Xiaoguang |
| 作者单位 | 1.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China 2.Natl Inst Biol Sci, Beijing, Peoples R China 3.Chinese Acad Med Sci, Peking Union Med Coll, Grad Program, Beijing 100730, Peoples R China 4.Tsinghua Univ, Sch Life Sci, Key Lab Prot Sci, Minist Educ, Beijing 100084, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc Chinese Acad Sc, Beijing, Peoples R China 6.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing 100871, Peoples R China 7.Peking Univ, Synthet & Funct Biomol Ctr, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tan, Dan,Li, Qiang,Zhang, Mei-Jun,et al. Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states[J]. ELIFE,2016,5:51. |
| APA | Tan, Dan.,Li, Qiang.,Zhang, Mei-Jun.,Liu, Chao.,Ma, Chengying.,...&Lei, Xiaoguang.(2016).Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states.ELIFE,5,51. |
| MLA | Tan, Dan,et al."Trifunctional cross-linker for mapping protein-protein interaction networks and comparing protein conformational states".ELIFE 5(2016):51. |
入库方式： OAI收割
来源：计算技术研究所
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