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pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3

文献类型：期刊论文

作者Zeng, Wen-Feng1,2; Liu, Ming-Qi3; Zhang, Yang3; Wu, Jian-Qiang1,2; Fang, Pan3; Peng, Chao4; Nie, Aiying5; Yan, Guoquan3; Cao, Weiqian3; Liu, Chao1
刊名SCIENTIFIC REPORTS
出版日期2016-05-03
卷号6页码:10
ISSN号2045-2322
DOI10.1038/srep25102
英文摘要Confident characterization of the microheterogeneity of protein glycosylation through identification of intact glycopeptides remains one of the toughest analytical challenges for glycoproteomics. Recently proposed mass spectrometry (MS)-based methods still have some defects such as lack of the false discovery rate (FDR) analysis for the glycan identification and lack of sufficient fragmentation information for the peptide identification. Here we proposed pGlyco, a novel pipeline for the identification of intact glycopeptides by using complementary MS techniques: 1) HCD-MS/MS followed by product-dependent CID-MS/MS was used to provide complementary fragments to identify the glycans, and a novel target-decoy method was developed to estimate the false discovery rate of the glycan identification; 2) data-dependent acquisition of MS3 for some most intense peaks of HCD-MS/MS was used to provide fragments to identify the peptide backbones. By integrating HCD-MS/MS, CID-MS/MS and MS3, intact glycopeptides could be confidently identified. With pGlyco, a standard glycoprotein mixture was analyzed in the Orbitrap Fusion, and 309 non-redundant intact glycopeptides were identified with detailed spectral information of both glycans and peptides.
资助项目National Basic Research Program of China[2011CB910600] ; National Basic Research Program of China[2013CB911203] ; National Basic Research Program of China[2010CB912701] ; National Basic Research Program of China[2011CB910604] ; National Natural Science Foundation of China[21227805] ; National Natural Science Foundation of China[31300680] ; Hi-Tech Research and Development Program of China[2014AA020902] ; Hi-Tech Research and Development Program of China[2012AA020203]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000375290500001
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.204/handle/2XEOYT63/8537]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Wong, Catherine C. L.; He, Si-Min; Yang, Pengyuan
作者单位1.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, Shanghai, Peoples R China
5.Thermo Fisher Sci Co Ltd, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Wen-Feng,Liu, Ming-Qi,Zhang, Yang,et al. pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3[J]. SCIENTIFIC REPORTS,2016,6:10.
APA Zeng, Wen-Feng.,Liu, Ming-Qi.,Zhang, Yang.,Wu, Jian-Qiang.,Fang, Pan.,...&Yang, Pengyuan.(2016).pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3.SCIENTIFIC REPORTS,6,10.
MLA Zeng, Wen-Feng,et al."pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD-and CID-MS/MS and MS3".SCIENTIFIC REPORTS 6(2016):10.

入库方式： OAI收割

来源：计算技术研究所

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