Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice
文献类型：期刊论文
| 作者 | McClatchy, Daniel B.2; Ma, Yuanhui2; Liu, Chao3; Stein, Benjamin D.2; Martinez-Bartolome, Salvador2; Vasquez, Debbie1; Hellberg, Kristina1; Shaw, Reuben J.1,4; Yates, John R., III2 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 14期号:11页码:4815-4822 |
| 关键词 | azidohomoalanine newly synthesized proteins proteomics mass spectrometry BONCAT LKB1 quantitation |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.5b00653 |
| 英文摘要 | Quantification of proteomes by mass spectrometry has proven to be useful to study human pathology recapitulated in cellular or animal models of disease. Enriching and quantifying newly synthesized proteins (NSPs) at set time points by mass spectrometry has the potential to identify important early regulatory or expression changes associated with disease states or perturbations. NSP can be enriched from proteomes by employing pulsed introduction of the non-canonical amino acid, azidohomoalanine (AHA). We demonstrate that pulsed introduction of AHA in the feed of mice can label and identify NSP from multiple tissues. Furthermore, we quantitate differences in new protein expression resulting from CRE-LOX initiated knockout of LKB1 in mouse livers. Overall, the PALM strategy allows for the first time in vivo labeling of mouse tissues to differentiate protein synthesis rates at discrete time points. |
| 资助项目 | National Institutes of Health[P41 GM103533] ; National Institutes of Health[R01 MH067880] ; National Institutes of Health[1 R01 MH100175] ; National Institutes of Health[HHSN268201000035C] ; National Institutes of Health[R01DK080425] ; National Key Basic Research and Development Program of China (973)[2012CB910602] ; George E. Hewitt Foundation for Medical Research |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000364435100033 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/9169]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Yates, John R., III |
| 作者单位 | 1.Salk Inst Biol Studies, Mol & Cell Biol Lab, La Jolla, CA 92037 USA 2.Scripps Res Inst, Dept Chem Physiol & Mol & Cellular Mol Biol, La Jolla, CA 92037 USA 3.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China 4.Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA |
| 推荐引用方式 GB/T 7714 | McClatchy, Daniel B.,Ma, Yuanhui,Liu, Chao,et al. Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(11):4815-4822. |
| APA | McClatchy, Daniel B..,Ma, Yuanhui.,Liu, Chao.,Stein, Benjamin D..,Martinez-Bartolome, Salvador.,...&Yates, John R., III.(2015).Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.JOURNAL OF PROTEOME RESEARCH,14(11),4815-4822. |
| MLA | McClatchy, Daniel B.,et al."Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice".JOURNAL OF PROTEOME RESEARCH 14.11(2015):4815-4822. |
入库方式： OAI收割
来源：计算技术研究所
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