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Experimental Validation of Bacillus anthracis A16R Proteogenomics

文献类型：期刊论文

作者Gao, Zhiqi2; Wang, Zhiqiang3,4,5; Zhang, Kun1; Li, Yanchang3; Zhang, Tao3; Wang, Dongshu2; Liu, Xiankai2; Feng, Erling2; Chang, Lei3; Xu, Junjie2
刊名SCIENTIFIC REPORTS
出版日期2015-10-01
卷号5页码:10
ISSN号2045-2322
DOI10.1038/srep14608
英文摘要Anthrax, caused by the pathogenic bacterium Bacillus anthracis, is a zoonosis that causes serious disease and is of significant concern as a biological warfare agent. Validating annotated genes and reannotating misannotated genes are important to understand its biology and mechanisms of pathogenicity. Proteomics studies are, to date, the best method for verifying and improving current annotations. To this end, the proteome of B. anthracis A16R was analyzed via one-dimensional gel electrophoresis followed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). In total, we identified 3,712 proteins, including many regulatory and key functional proteins at relatively low abundance, representing the most complete proteome of B. anthracis to date. Interestingly, eight sequencing errors were detected by proteogenomic analysis and corrected by resequencing. More importantly, three unannotated peptide fragments were identified in this study and validated by synthetic peptide mass spectrum mapping and green fluorescent protein fusion experiments. These data not only give a more comprehensive understanding of B. anthracis A16R but also demonstrate the power of proteomics to improve genome annotations and determine true translational elements.
资助项目Key Project of China Programs for Communicable Diseases[2012ZX10004215-001-002] ; Key Project of China Programs for Drug Development[2013ZX09304101] ; National Natural Science Foundation of China[81125012] ; National Natural Science Foundation of China[81271785] ; National Natural Science Foundation of China[81471912]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000362082600001
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.204/handle/2XEOYT63/9301]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者Xu, Ping
作者单位1.Chinese Acad Sci, Inst Comp Technol, Beijing 100190, Peoples R China
2.Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
3.Beijing Inst Radiat Med, Beijing Proteome Res Ctr, Natl Engn Res Ctr Prot Drugs, State Key Lab Prote,Natl Ctr Prot Sci, Beijing 100850, Peoples R China
4.Wuhan Univ, Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Peoples R China
5.Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714		 Gao, Zhiqi,Wang, Zhiqiang,Zhang, Kun,et al. Experimental Validation of Bacillus anthracis A16R Proteogenomics[J]. SCIENTIFIC REPORTS,2015,5:10.
APA Gao, Zhiqi.,Wang, Zhiqiang.,Zhang, Kun.,Li, Yanchang.,Zhang, Tao.,...&Wang, Hengliang.(2015).Experimental Validation of Bacillus anthracis A16R Proteogenomics.SCIENTIFIC REPORTS,5,10.
MLA Gao, Zhiqi,et al."Experimental Validation of Bacillus anthracis A16R Proteogenomics".SCIENTIFIC REPORTS 5(2015):10.

入库方式： OAI收割

来源：计算技术研究所

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