Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray
文献类型：期刊论文
| 作者 | He, Housheng; Cai, Lun; Skogerbo, Geir; Deng, Wei; Liu, Tao; Zhu, Xiaopeng; Wang, Yudong; Jia, Dong; Zhang, Zhihua; Tao, Yong |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2006 |
| 卷号 | 34期号:10页码:2976-2983 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkl371 |
| 英文摘要 | Small non-coding RNAs (ncRNAs) are encoded by genes that function at the RNA level, and several hundred ncRNAs have been identified in various organisms. Here we describe an analysis of the small non-coding transcriptome of Caenorhabditis elegans, microRNAs excepted. As a substantial fraction of the ncRNAs is located in introns of protein-coding genes in C.elegans, we also analysed the relationship between ncRNA and host gene expression. To this end, we designed a combined microarray, which included probes against ncRNA as well as host gene mRNA transcripts. The microarray revealed pronounced differences in expression profiles, even among ncRNAs with housekeeping functions (e.g. snRNAs and snoRNAs), indicating distinct developmental regulation and stage-specific functions of a number of novel transcripts. Analysis of ncRNA-host mRNA relations showed that the expression of intronic ncRNA loci with conserved upstream motifs was not correlated to (and much higher than) expression levels of their host genes. Even promoter-less intronic ncRNA loci, though showing a clear correlation to host gene expression, appeared to have a surprising amount of,expressional freedom', depending on host gene function. Taken together, our microarray analysis presents a more complete and detailed picture of a non-coding transcriptome than hitherto has been presented for any other multicellular organism. |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000239430900013 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/10501]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Chen, Runsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Natl Lab Biomacromol, Inst Biophys, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Computat Biol Res Grp, Div Intelligent Software Syst, Beijing 100080, Peoples R China 4.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China 5.Univ Tennessee, Hlth Sci Ctr, Dept Mol Sci, Ctr Gen & Bioinformat, Memphis, TN 38163 USA 6.Chinese Acad Sci, Grad Sch, Beijing 100080, Peoples R China 7.Chinese Acad Sci, Beijing Gen Inst, Beijing 101300, Peoples R China 8.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Housheng,Cai, Lun,Skogerbo, Geir,et al. Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray[J]. NUCLEIC ACIDS RESEARCH,2006,34(10):2976-2983. |
| APA | He, Housheng.,Cai, Lun.,Skogerbo, Geir.,Deng, Wei.,Liu, Tao.,...&Chen, Runsheng.(2006).Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray.NUCLEIC ACIDS RESEARCH,34(10),2976-2983. |
| MLA | He, Housheng,et al."Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray".NUCLEIC ACIDS RESEARCH 34.10(2006):2976-2983. |
入库方式： OAI收割
来源：计算技术研究所
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