Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Jia, Dong1,2; Cai, Lun3,4; He, Housheng1,4; Skogerbo, Geir1; Li, Tiantian1,4; Aftab, Muhammad Nauman1,4; Chen, Runsheng1 |
| 刊名 | BMC MOLECULAR BIOLOGY
 |
| 出版日期 | 2007-09-29 |
| 卷号 | 8页码:9 |
| ISSN号 | 1471-2199 |
| DOI | 10.1186/1471-2199-8-86 |
| 英文摘要 | Background: The 2,2,7-trimethylguanosine (TMG) cap structure is an important functional characteristic of ncRNAs with critical cellular roles, such as some snRNAs. Here we used immunoprecipitation with both K121 and R1131 anti-TMG antibodies to systematically identify the TMG cap structures for all presently characterized ncRNAs in C. elegans. Results: The two anti-TMG antibodies precipitated a similar group of the C. elegans ncRNAs. All snRNAs known to have a TMG cap structure were found in the precipitate, indicating that our identification system was efficient. Other ncRNA families related to splicing, such as SL RNAs and Sm Y RNAs, were also found in the precipitate, as were 7 C/D box snoRNAs. Further analysis showed that the SL RNAs and the Sm Y RNAs shared a very similar Sm binding site element ( AAU(4)-(5)GGA), which sequence composition differed somewhat from those of other U snRNAs. There were also 16 ncRNAs without an Sm binding site element in the precipitate, suggesting that for these ncRNAs, TMG formation may occur independently of Sm proteins. Conclusion: Our results showed that most ncRNAs predicted to be transcribed by RNA polymerase II had a TMG cap, while those predicted to be transcribed by RNA plymerase III or located in introns did not have a TMG cap structure. Compared to ncRNAs without a TMG cap, TMG-capped ncRNAs tended to have higher expression levels. Five functionally non-annotated ncRNAs also have a TMG cap structure, which might be helpful for identifying the cellular roles of these ncRNAs. |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000252379900001 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/10894]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Chen, Runsheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Bioinformat Lab, Beijing 100101, Peoples R China 2.Shanxi Agr Univ, Taigu 030801, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100080, Peoples R China 4.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia, Dong,Cai, Lun,He, Housheng,et al. Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans[J]. BMC MOLECULAR BIOLOGY,2007,8:9. |
| APA | Jia, Dong.,Cai, Lun.,He, Housheng.,Skogerbo, Geir.,Li, Tiantian.,...&Chen, Runsheng.(2007).Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans.BMC MOLECULAR BIOLOGY,8,9. |
| MLA | Jia, Dong,et al."Systematic identification of non-coding RNA 2,2,7-trimethylguanosine cap structures in Caenorhabditis elegans".BMC MOLECULAR BIOLOGY 8(2007):9. |
入库方式： OAI收割
来源：计算技术研究所
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