PeakSelect: preprocessing tandem mass spectra for better peptide identification
文献类型：期刊论文
| 作者 | Zhang, Jingfen1; He, Simin1; Ling, Charles X.2; Ca, Xingjun3; Zeng, Rong3; Gao, Wen1 |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 22期号:8页码:1203-1212 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.3488 |
| 英文摘要 | We present a new preprocessing method, PeakSelect, to improve the accuracy and efficiency of Tandem Mass-Spec peptide (protein) identification. The fundamental difference between noise and fragment ions in spectra is that ions have isotopes but noise does not. We propose a new and important concept of an Isotope Pattern Vector (IPV) which characterizes the isotope cluster of fragment ions. Then the noise and real peaks can be distinguished by the quantitative IPV values. PeakSelect first uses a new method of the Gaussian Mixture Model and Expectation-Maximization (EM) algorithm to find the base intensity level (baseline) in a spectrum. Then PeakSelect selects features based on the IPV and baseline, and constructs a decision tree to automatically classify the peaks into different categories such as noise, single ion peaks, and overlapping peaks. Experiments show that PeakSelect can help to reduce the Mascot searching time and increase the reliability of peptide identifications. In particular, PeakSelect performs well on complex spectra with a large number of peaks from large peptides, and supports more sequence identification than other well-known systems. Copyright (c) 2008 John Wiley & Sons, Ltd. |
| WOS研究方向 | Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000255362300016 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/11072]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Zhang, Jingfen |
| 作者单位 | 1.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China 2.Univ Western Ontario, Dept Comp Sci, London, ON, Canada 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jingfen,He, Simin,Ling, Charles X.,et al. PeakSelect: preprocessing tandem mass spectra for better peptide identification[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2008,22(8):1203-1212. |
| APA | Zhang, Jingfen,He, Simin,Ling, Charles X.,Ca, Xingjun,Zeng, Rong,&Gao, Wen.(2008).PeakSelect: preprocessing tandem mass spectra for better peptide identification.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,22(8),1203-1212. |
| MLA | Zhang, Jingfen,et al."PeakSelect: preprocessing tandem mass spectra for better peptide identification".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 22.8(2008):1203-1212. |
入库方式： OAI收割
来源：计算技术研究所
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