Isotope pattern vector based tandem mass spectral data calibration for improved peptide and protein identification
文献类型：期刊论文
| 作者 | Zhang, Jingfen; Xu, Dong1; Gao, Wen3; Lin, Guohui2; He, Simin3 |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 23期号:21页码:3448-3456 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.4272 |
| 英文摘要 | Tandem mass spectra contain noisy peaks which make peak picking for peptide identification difficult. Moreover, all spectral peaks can be shifted due to systematic measurement errors. In this paper, a novel use of an isotope pattern vector (IPV) is proposed for denoising and systematic measurement error prediction. By matching the experimental IPVs with the theoretical IPVs of candidate fragment ions, true ionic peaks can be identified. Furthermore, these identified experimental IPVs and their corresponding theoretical IPVs are used in an optimization process to predict the systematic measurement error associated with the target spectrum. In return, the subsequent spectral data calibration based on the predicted systematic measurement error enhances the data quality. We show that such an integrated denoising and calibration process leads to significantly improved peptide and protein identification. Different from the commonly employed chemical calibration methods, our IPV-based method is a purely computational method for individual spectra analysis and globally optimizes the use of spectral data. Copyright (C) 2009 John Wiley & Sons, Ltd. |
| 资助项目 | MU-Monsanto Program ; NSF[NSF/ITRIIS-0407204] ; NSERC ; National Key Basic Research and Development Program (973)[2002CB713807] ; National High Technology Research and Development Program (863)[2007AA02Z315] ; National High Technology Research and Development Program (863)[2007AA02Z326] |
| WOS研究方向 | Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000271431500012 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/11939]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Lin, Guohui |
| 作者单位 | 1.Univ Missouri, Dept Comp Sci, Columbia, MO 65211 USA 2.Univ Alberta, Dept Comp Sci, Edmonton, AB T6G 2E8, Canada 3.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jingfen,Xu, Dong,Gao, Wen,et al. Isotope pattern vector based tandem mass spectral data calibration for improved peptide and protein identification[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2009,23(21):3448-3456. |
| APA | Zhang, Jingfen,Xu, Dong,Gao, Wen,Lin, Guohui,&He, Simin.(2009).Isotope pattern vector based tandem mass spectral data calibration for improved peptide and protein identification.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,23(21),3448-3456. |
| MLA | Zhang, Jingfen,et al."Isotope pattern vector based tandem mass spectral data calibration for improved peptide and protein identification".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 23.21(2009):3448-3456. |
入库方式： OAI收割
来源：计算技术研究所
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