Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy
文献类型：期刊论文
| 作者 | Liu Ying1,2; He Jiu-Ming1,2; Sun Rui-Xiang3; Liu Chao3; Zhang Rui-Ping1,2; Shi Jian-Gong1,2; Zeper, Abliz1,2 |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 39期号:3页码:323-329 |
| 关键词 | Nuclear magnetic resonance/liquid chromatography/mass spectrometry parallel dynamic spectroscopy Incomplete separation strategy Simultaneous structural identification in mixture Forsythia Suspensa Phenylethanoid glycosides |
| ISSN号 | 0253-3820 |
| DOI | 10.3724/SP.J.1096.2011.00323 |
| 英文摘要 | Based on an incomplete separation strategy, the crude extract of Forsythia Suspensa was separated into a series of fractions with the concentration of constituent dynamic variation by RP preparative chromatography. NMR/LC-MS parallel dynamic spectroscopic (PDS) method was applied to identify the phenylethanoid glycosides in these fractions. The complementary spectral information of the same individual constituent in the crude extract was simultaneously obtained from the spectra of mixtures, including retention time (R(t)), mass/charge (m/z) and chemical shift (delta). Seven constituents were identified successfully, including a new phenylethanoid glycoside and a novel Dimeric-2-(4-aminophenyl) ethyl-beta-D-glucopyranoside which is the first example of dimeric phenylethanol alkaloid glycoside. The results demonstrated that NMR/LC-MS PDS can be used to identify the constituents with more complicated structures from herbs and their active extracts. Especially, this method is suitable for analysis and identification of minimum-quantity constituents and constituents with similar polarity which are hardly obtained in routine experimental conditions. In addition, this method has the advantage of rapid discovering and identifying new compounds from complex mixtures, which can greatly improve the analytical efficiency. |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000290841800006 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/12912]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Zeper, Abliz |
| 作者单位 | 1.Chinese Acad Med Sci, Inst Mat Med, Key Lab Bioact Subst & Resource Utilizat Chinese, Minist Educ, Beijing 100050, Peoples R China 2.Peking Union Med Coll, Beijing 100050, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Ying,He Jiu-Ming,Sun Rui-Xiang,et al. Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(3):323-329. |
| APA | Liu Ying.,He Jiu-Ming.,Sun Rui-Xiang.,Liu Chao.,Zhang Rui-Ping.,...&Zeper, Abliz.(2011).Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(3),323-329. |
| MLA | Liu Ying,et al."Simultaneous Structural Identification of Constituents in Active Herbal Extract of Forsythia Suspensa Using Nuclear Magnetic Resonance/Liquid Chromatography-Mass Spectrometry Parallel Dynamic Spectroscopy".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.3(2011):323-329. |
入库方式： OAI收割
来源：计算技术研究所
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