Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using H-1 NMR/RRLC-MS Parallel Dynamic Spectroscopy
文献类型：期刊论文
| 作者 | Wang, Xiao-Xue1,2; He, Jiu-Ming1,2; Wang, Chun-Lan3,4; Zhang, Rui-Ping1,2; He, Wen-Yi1,2; Guo, Shun-Xing3,4; Sun, Rui-Xiang5; Abliz, Zeper1,2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 12期号:4页码:2556-2571 |
| 关键词 | H-1 NMR/RRLC-MS parallel dynamic spectroscopy flavonoids organic acids crude extract Anoectochilus roburghii |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms12042556 |
| 英文摘要 | Nuclear magnetic resonance/liquid chromatography-mass spectroscopy parallel dynamic spectroscopy (NMR/LC-MS PDS) is a method aimed at the simultaneous structural identification of natural products in complex mixtures. In this study, the method is illustrated with respect to H-1 NMR and rapid resolution liquid chromatography-mass spectroscopy (RRLC-MS) data, acquired from the crude extract of Anoectochilus roxburghii, which was separated into a series of fractions with the concentration of constituent dynamic variation using reversed-phase preparative chromatography. Through fraction ranges and intensity changing profiles in H-1 NMR/RRLC-MS PDS spectrum, 1H NMR and the extracted ion chromatogram (XIC) signals deriving from the same individual constituent, were correlated due to the signal amplitude co-variation resulting from the concentration variation of constituents in a series of incompletely separated fractions. H-1 NMR/RRLC-MS PDS was then successfully used to identify three types of natural products, including eight flavonoids, four organic acids and p-hydroxybenzaldehyde, five of which have not previously been reported in Anoectochilus roxburghii. In addition, two groups of co-eluted compounds were successfully identified. The results prove that this approach should be of benefit in the unequivocal structural determination of a variety of classes of compounds from extremely complex mixtures, such as herbs and biological samples, which will lead to improved efficiency in the identification of new potential lead compounds. |
| 资助项目 | National Key Technologies R & D Program for New Drugs[2009ZX09301-003-7-1] ; National Natural Scientific Foundation of China[20775091] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000289974600007 |
| 出版者 | MDPI AG |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/13018]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Abliz, Zeper |
| 作者单位 | 1.Chinese Acad Med Sci, Inst Mat Med, Minist Educ, Key Lab Bioact Subst & Resource Utilizat Chinese, Beijing 100050, Peoples R China 2.Peking Union Med Coll, Beijing 100050, Peoples R China 3.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100094, Peoples R China 4.Peking Union Med Coll, Beijing 100094, Peoples R China 5.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiao-Xue,He, Jiu-Ming,Wang, Chun-Lan,et al. Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using H-1 NMR/RRLC-MS Parallel Dynamic Spectroscopy[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2011,12(4):2556-2571. |
| APA | Wang, Xiao-Xue.,He, Jiu-Ming.,Wang, Chun-Lan.,Zhang, Rui-Ping.,He, Wen-Yi.,...&Abliz, Zeper.(2011).Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using H-1 NMR/RRLC-MS Parallel Dynamic Spectroscopy.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,12(4),2556-2571. |
| MLA | Wang, Xiao-Xue,et al."Simultaneous Structural Identification of Natural Products in Fractions of Crude Extract of the Rare Endangered Plant Anoectochilus roxburghii Using H-1 NMR/RRLC-MS Parallel Dynamic Spectroscopy".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 12.4(2011):2556-2571. |
入库方式： OAI收割
来源：计算技术研究所
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