A draft sequence of the rice (Oryza sativa ssp indica) genome
文献类型：期刊论文
| 作者 | Yu, J; Hu, SN; Wang, J; Li, SG; Wong, KSG; Liu, B; Deng, Y; Dai, L; Zhou, Y; Zhang, XQ |
| 刊名 | CHINESE SCIENCE BULLETIN
 |
| 出版日期 | 2001-12-01 |
| 卷号 | 46期号:23页码:1937-1942 |
| ISSN号 | 1001-6538 |
| 关键词 | rice genome draft sequences |
| 英文摘要 | The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have produced a draft rice genome sequence of Oryza sativa ssp. indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEMBLER, a custom-designed software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences from both indica and japanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0% of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities to facilitate genomic and genetic studies on rice biology. |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | SCIENCE CHINA PRESS |
| WOS记录号 | WOS:000173381900001 |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/13372]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Yang, HM |
| 作者单位 | 1.Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Ctr Genom & Bioinformat, Beijing Genom Inst, Beijing 101300, Peoples R China 4.Zhejiang Univ, Hangzhou Genom Inst, Inst Bioinformat, Key Lab Bioinformat, Hangzhou 310007, Peoples R China 5.Natl Hybrid Rice R&D Ctr, Changsha 410125, Peoples R China 6.Chinese Acad Sci, Inst Biophys, Lab Bioinformat, Beijing 100101, Peoples R China 7.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 8.Chinese Acad Sci, Inst Theoret Phys, Beijing 100080, Peoples R China 9.Digital China Ltd, Beijing 100080, Peoples R China 10.Xi An Jiao Tong Univ, Coll Med, Xian 710061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, J,Hu, SN,Wang, J,et al. A draft sequence of the rice (Oryza sativa ssp indica) genome[J]. CHINESE SCIENCE BULLETIN,2001,46(23):1937-1942. |
| APA | Yu, J.,Hu, SN.,Wang, J.,Li, SG.,Wong, KSG.,...&Yang, HM.(2001).A draft sequence of the rice (Oryza sativa ssp indica) genome.CHINESE SCIENCE BULLETIN,46(23),1937-1942. |
| MLA | Yu, J,et al."A draft sequence of the rice (Oryza sativa ssp indica) genome".CHINESE SCIENCE BULLETIN 46.23(2001):1937-1942. |
入库方式： OAI收割
来源：计算技术研究所
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