Dehydroascorbate Reductase and Glutathione Reductase Play an Important Role in Scavenging Hydrogen Peroxide during Natural and Artificial Dehydration of Jatropha curcas Seeds
文献类型：期刊论文
| 作者 | Xu, ZF
|
| 刊名 | JOURNAL OF PLANT BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 55期号:6页码:469-480 |
| 关键词 | dehydroascorbate reductase glutathione reductase hydrogen peroxide Jatropha curcas scavenging |
| 通讯作者 | Lee, CH |
| 中文摘要 | Changes in H2O2, and the main antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR) and glutathione reductase (GR), in endospermic and embryonic tissues were studied in developing and artificially dried Jatropha curcas seeds. Immature seeds were desiccation-tolerant at 80 days after flowering, as they were able to germinate fully after artificial drying on silica gel had reduced their water content to 10-12% of fresh weight. In both endospermic and embryonic tissues, H2O2 level and, consequently, lipid peroxide content, decreased during seed development as well as after artificial dehydration of developing seeds. All examined antioxidant enzymes except DHAR showed a decrease in total activity in mature stages as compared with early stages. Expression analysis of SOD genes revealed that the decrease in total SOD activities was related to the decrease in Cu/Zn-SOD expression, while the continuous activity of SOD during maturation was related to an increase in Mn-SOD expression. Artificial drying resulted in increased SOD and DHAR activity, irrespective of the developmental stage. Our results revealed weak participation of CAT and APX in H2O2 scavenging, as well as no significant alterations in GR activities either during maturation or after artificial drying. Changes in SOD and GR isoenzyme patterns occurred during maturation-related drying, but not after artificial drying. These results highlight the role of ascorbate-glutathione cycle enzymes (DEAR and GR) in H2O2 scavenging during maturation or after artificial drying of developing J. curcas seeds. |
| 公开日期 | 2013-01-17 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/3887]  |
| 专题 | 西双版纳热带植物园_能源植物分子育种研究组 |
| 推荐引用方式 GB/T 7714 | Xu, ZF. Dehydroascorbate Reductase and Glutathione Reductase Play an Important Role in Scavenging Hydrogen Peroxide during Natural and Artificial Dehydration of Jatropha curcas Seeds[J]. JOURNAL OF PLANT BIOLOGY,2012,55(6):469-480. |
| APA | Xu, ZF.(2012).Dehydroascorbate Reductase and Glutathione Reductase Play an Important Role in Scavenging Hydrogen Peroxide during Natural and Artificial Dehydration of Jatropha curcas Seeds.JOURNAL OF PLANT BIOLOGY,55(6),469-480. |
| MLA | Xu, ZF."Dehydroascorbate Reductase and Glutathione Reductase Play an Important Role in Scavenging Hydrogen Peroxide during Natural and Artificial Dehydration of Jatropha curcas Seeds".JOURNAL OF PLANT BIOLOGY 55.6(2012):469-480. |
入库方式： OAI收割
来源：西双版纳热带植物园
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