Solution structure and biological activity of recombinant salmon calcitonin S-sulfonated analog
文献类型：期刊论文
| 作者 | Wang YF(王跃丰) ; Dou H(窦洪) ; Cao CY(曹春阳) ; Zhang NX(张乃霞) ; Ma JB(麻锦彪) ; Mao JF(毛积芳) ; Wu HM(吴厚铭) |
| 刊名 | Biochem. Biophys. Res. Commun.
 |
| 出版日期 | 2003 |
| 卷号 | 306期号:2页码:582-589 |
| ISSN号 | 0006-291X |
| 其他题名 | 重组降鲑鱼钙素S-磺酸化类似物的溶液结构和生物活性 |
| 通讯作者 | 吴厚铭 |
| 英文摘要 | Salmon calcitonin S-sulfonated analog (abbreviated as [S-SO3-]rsCT) was prepared by introducing two sulfonic groups into the side chains of Cysl and Cys7 of recombinant salmon calcitonin. The hypocalcemic potency of this open-chain analog is 5500 IU/mg, which is about 30'X, higher than that (4500 IU/mg) of the wild type. The solution conformation of [S-SO3 ]rsCT was studied in aqueous trifinoroethanol solution by CD. 2D-NMR spectroscopy. And distance geometry calculations. In the mixture of 60'X, TFE and 40'/. Water, the peptide assumes an amphipathic α-helix in the region of residues 4-22. which is one turn longer than that of the native sCT. The structural feature analysis of the peptide revealed the presence of hydrophobic surface composed of five hydro-phobic side chains of residues Leu4, Leu9, Leul2. Leul6. and Leul9. and a network of salt-bridges that consisted of a tetrad of oppositely charged side chains (Cys7-SO3 -Lys 11+-Glul5-Lysl8+). The multiple salt bridges resulted in the stabilization of the longer amphipathic α-helix. Meanwhile, the higher hypocalcemic potency of the peptide could be attributed to the array of hydrophobic side chains of five leucine residues of the amphipathic α-helix |
| 学科主题 | 天然产物有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/S0006-291X(03)01028-3 |
| 语种 | 英语 |
| WOS记录号 | WOS:000183734800041 |
| 公开日期 | 2013-02-21 |
| 源URL | [http://202.127.28.38/handle/331003/17327]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Wang YF,Dou H,Cao CY,et al. Solution structure and biological activity of recombinant salmon calcitonin S-sulfonated analog[J]. Biochem. Biophys. Res. Commun.,2003,306(2):582-589. |
| APA | 王跃丰.,窦洪.,曹春阳.,张乃霞.,麻锦彪.,...&吴厚铭.(2003).Solution structure and biological activity of recombinant salmon calcitonin S-sulfonated analog.Biochem. Biophys. Res. Commun.,306(2),582-589. |
| MLA | 王跃丰,et al."Solution structure and biological activity of recombinant salmon calcitonin S-sulfonated analog".Biochem. Biophys. Res. Commun. 306.2(2003):582-589. |
入库方式： OAI收割
来源：上海有机化学研究所
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