Interaction sites between the Slo1 pore and the NH2 terminus of the beta(2) subunit, probed with a three-residue sensor
文献类型：期刊论文
| 作者 | Li H(李辉) ; Yao J(姚镜) ; Tong XT(童孝田) ; Guo ZH(郭兆华) ; Wu Y(吴英) ; Sun L(孙亮) ; Pan N(潘娜) ; Wu HM(吴厚铭) ; Xu T(徐滔) ; Ding JP(丁久平) |
| 刊名 | J. Biol. Chem.
 |
| 出版日期 | 2007 |
| 卷号 | 282期号:24页码:17720-17728 |
| ISSN号 | 0021-9258 |
| 其他题名 | 用三残基探针探测钾离子通道Slo1孔道与第二亚单元NH2末端相互作用位点 |
| 通讯作者 | 丁久平 |
| 英文摘要 | Calcium- and voltage-gated (BK) K+ channels encoded by Slo1 play an essential role in nervous systems. Although it shares many common features with voltage-dependent KV channels, the BK channel exhibits differences in gating and inactivation. Using a mutant in which FWI replaces three residues (FIW) in the NH2 terminus of wild-type beta 2-subunits, in conjunction with alanine-scanning mutagenesis of the Slo1 S6 segment, we identify that the NH2 terminus of beta 2-subunits interacts with the residues near the cytosolic superficial mouth of BK channels during inactivation. The cytosolic blockers did not share the sites with NH2 terminus of beta 2-subunits. A novel blocking-inactivating scheme was proposed to account for the observed noncompetition inactivation. Our results also suggest that the residue Ile-323 plays a dual role in interacting with the NH2 terminus of beta 2-subunits and modulating the gating of BK channels. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1074/jbc.M607063200 |
| 语种 | 英语 |
| WOS记录号 | WOS:000247084500045 |
| 公开日期 | 2013-02-21 |
| 源URL | [http://202.127.28.38/handle/331003/17381]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Li H,Yao J,Tong XT,et al. Interaction sites between the Slo1 pore and the NH2 terminus of the beta(2) subunit, probed with a three-residue sensor[J]. J. Biol. Chem.,2007,282(24):17720-17728. |
| APA | 李辉.,姚镜.,童孝田.,郭兆华.,吴英.,...&丁久平.(2007).Interaction sites between the Slo1 pore and the NH2 terminus of the beta(2) subunit, probed with a three-residue sensor.J. Biol. Chem.,282(24),17720-17728. |
| MLA | 李辉,et al."Interaction sites between the Slo1 pore and the NH2 terminus of the beta(2) subunit, probed with a three-residue sensor".J. Biol. Chem. 282.24(2007):17720-17728. |
入库方式： OAI收割
来源：上海有机化学研究所
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