Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States
文献类型：期刊论文
| 作者 | Lan WX(蓝文贤) ; WANG ZHONGHUA ; Yang ZZ(杨中正) ; Zhu J(朱静) ; YING TIANLEI ; JIANG XIANWANG ; ZHANG XU ; Wu HM(吴厚铭) ; Liu ML(刘买利) ; TAN XIANGSHI ; Cao CY(曹春阳) ; HUANG ZHONGXIAN |
| 刊名 | PLoS One
 |
| 出版日期 | 2011 |
| 卷号 | 6期号:11页码:e27219 |
| ISSN号 | 1932-6203 |
| 其他题名 | 酵母iso-1型细胞色素c在氧化态和还原态时构象的转变 |
| 通讯作者 | 曹春阳 ; HUANG ZHONGXIAN |
| 英文摘要 | To convert cyt c into a peroxidase-like metalloenzyme, the P71H mutant was designed to introduce a distal histidine. Unexpectedly, its peroxidase activity was found even lower than that of the native, and that the axial ligation of heme iron was changed to His71/His18 in the oxidized state, while to Met80/His18 in the reduced state, characterized by UV-visible, circular dichroism, and resonance Raman spectroscopy. To further probe the functional importance of Pro71 in oxidation state dependent conformational changes occurred in cyt c, the solution structures of P71H mutant in both oxidation states were determined. The structures indicate that the half molecule of cyt c (aa 50-102) presents a kind of "zigzag riveting ruler" structure, residues at certain positions of this region such as Pro71, Lys73 can move a big distance by altering the tertiary structure while maintaining the secondary structures. This finding provides a molecular insight into conformational toggling in different oxidation states of cyt c that is principle significance to its biological functions in electron transfer and apoptosis. Structural analysis also reveals that Pro71 functions as a key hydrophobic patch in the folding of the polypeptide of the region (aa 50-102), to prevent heme pocket from the solvent. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1371/journal.pone.0027219 |
| 语种 | 英语 |
| WOS记录号 | WOS:000297349700020 |
| 公开日期 | 2013-02-21 |
| 源URL | [http://202.127.28.38/handle/331003/17409]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Lan WX,WANG ZHONGHUA,Yang ZZ,et al. Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States[J]. PLoS One,2011,6(11):e27219. |
| APA | 蓝文贤.,WANG ZHONGHUA.,杨中正.,朱静.,YING TIANLEI.,...&HUANG ZHONGXIAN.(2011).Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States.PLoS One,6(11),e27219. |
| MLA | 蓝文贤,et al."Conformational Toggling of Yeast Iso-1-Cytochrome c in the Oxidized and Reduced States".PLoS One 6.11(2011):e27219. |
入库方式： OAI收割
来源：上海有机化学研究所
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