Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst
文献类型：期刊论文
| 作者 | YU HUILEI ; Xu JH(许建和) ; Lu WY(卢文芽) ; Lin GQ(林国强) |
| 刊名 | J. Biotechnol.
 |
| 出版日期 | 2008 |
| 卷号 | 133期号:4页码:469-477 |
| ISSN号 | 0168-1656 |
| 其他题名 | Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst |
| 通讯作者 | 许建和 ; 林国强 |
| 英文摘要 | Salidroside is a natural glycoside with pharmacological activities of resisting anoxia, microwave radiation and fatigue, improving oxygen lack, and postponing ageing. In this work, salidroside and other natural glucosides such as cinnamyl O-beta-D-glucopyranoside and 4-methoxybenzyl O-beta-D-glucopyranoside were efficiently synthesized via an environmentally benign and energy economic process. In the synthetic process, apple seed, easily available from discards of fruit processing factories, was employed as a natural and green catalyst. Moreover, all of the catalyst, solvent and excessive substrate was reused or recycled. The biocatalytic reaction was carried out in a clean and less toxic medium of aqueous tert-butanol and the glucoside produced was selectively removed from reaction mixture by alumina column adsorption, making excessive substrate (aglycon) recyclable for a repeated use in the next batch of reaction. For improvement of the biocatalyst stability, apple seed meal was further cross-linked by glutaraldehyde, yielding a net-like porous structure within which the dissociating proteins were immobilized, resulting in improved permeability of the biocatalyst. After the simple cross-linking treatment, the half-life of apple seed catalyst was significantly improved from 29 days to 51 days. The productivity of the bioreactor in the case of salidroside can reach ca. 1.9 g l(-1) d(-1), affording the product in up to 99.3% purity after refinement. (C) 2007 Elsevier B.V. All rights reserved. |
| 学科主题 | 天然产物有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.jbiotec.2007.12.003 |
| 语种 | 英语 |
| WOS记录号 | WOS:000253875500008 |
| 公开日期 | 2013-02-20 |
| 源URL | [http://202.127.28.38/handle/331003/16681]  |
| 专题 | 上海有机化学研究所_中科院天然产物有机化学重点实验室 |
| 推荐引用方式 GB/T 7714 | YU HUILEI,Xu JH,Lu WY,et al. Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst[J]. J. Biotechnol.,2008,133(4):469-477. |
| APA | YU HUILEI,许建和,卢文芽,&林国强.(2008).Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst.J. Biotechnol.,133(4),469-477. |
| MLA | YU HUILEI,et al."Environmentally benign synthesis of natural glycosides using apple seed meal as green and robust biocatalyst".J. Biotechnol. 133.4(2008):469-477. |
入库方式： OAI收割
来源：上海有机化学研究所
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