Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish
文献类型：期刊论文
| 作者 | Wu, Nan; Li, Chang-Jian; Gui, Jian-Fang |
| 刊名 | BIOCHEMICAL GENETICS
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 47期号:11-12页码:749-762 |
| 关键词 | Oogenesis Spermatogenesis Sperm decondensation Carassius auratus gibelio Carassius auratus |
| ISSN号 | 0006-2928 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish. |
| 英文摘要 | Nucleophosmin/nucleoplasmin has been studied mostly in mammals and amphibians. To clarify the characteristics and function of nucleophosmin/nucleoplasmin in teleost fish, we cloned a full-length cDNA sequence from two cyprinid fish, Carassius auratus gibelio and Carassius auratus. Molecular characterization and multiple sequence alignments suggested that they are the homologs of nucleophosmin. RT-PCR and Western blot detected a specific expression in gonads, and immunofluorescence localization revealed their distribution in oogenic and spermatogenic cells. Furthermore, a sperm decondensation function was demonstrated by immunodepletion and in vitro sperm decondensation experiments. The data suggest that the cloned nucleophosmin should share expressional and functional characterization with nucleoplasmin and therefore provide novel evidence for a functional commonality of nucleophosmin and nucleoplasmin in fish. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Genetics & Heredity |
| 类目[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 关键词[WOS] | CARASSIUS-AURATUS-GIBELIO ; SILVER CRUCIAN CARP ; CHROMATIN DECONDENSATION ; SPERM DECONDENSATION ; XENOPUS-LAEVIS ; NUCLEOPLASMIN ; PROTEIN ; RNA ; NUCLEOPHOSMIN ; EXPRESSION |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N020]; Open Project of State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007]; Innovation Project of the Institute of Hydrobiology, Chinese Academy of Sciences [075A011301] |
| 语种 | 英语 |
| WOS记录号 | WOS:000272015400001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7480]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Nan,Li, Chang-Jian,Gui, Jian-Fang. Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish[J]. BIOCHEMICAL GENETICS,2009,47(11-12):749-762. |
| APA | Wu, Nan,Li, Chang-Jian,&Gui, Jian-Fang.(2009).Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish.BIOCHEMICAL GENETICS,47(11-12),749-762. |
| MLA | Wu, Nan,et al."Molecular Characterization and Functional Commonality of Nucleophosmin/Nucleoplasmin in Two Cyprinid Fish".BIOCHEMICAL GENETICS 47.11-12(2009):749-762. |
入库方式： OAI收割
来源：水生生物研究所
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