Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis
文献类型：期刊论文
| 作者 | Wei, Yanhong1; Shi, Xiongjie2; Zhang, Hongxia1; Wang, Jianshe1; Zhou, Bingsheng2; Dai, Jiayin1 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2009-10-19 |
| 卷号 | 95期号:1页码:27-36 |
| ISSN号 | 0166-445X |
| 关键词 | Polyfluorinated and perfluorinated compounds Toxicogenomics Combined toxicity Primary cultured hepatocytes Rare minnow (Gobiocypris rarus) |
| 通讯作者 | Dai, JY, Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China |
| 中文摘要 | Polyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | Polyfluorinated and perfluorinated compounds (PFCs) are used in numerous commercial products and have been ubiquitously detected in the environment as well as in the blood of humans and wildlife. To assess the combined effects caused by PFCs in mixtures, gene expression profiles were generated using a custom cDNA microarray to detect changes in primary cultured hepatocytes of rare minnows exposed to six individual PFCs (perfluorooctanoic acid, perfluorononanoic acid, perfluorodecanoic acid, perfluorododecanoic acid, perfluorooctane sulfonate, and 8:2 fluorotelomer alcohol) and four formulations of the PFCs mixtures. Mixtures as well as individual compounds consistently regulated a particular gene set, which suggests that these conserved genes may play a central role in the toxicity mediated by PFCs. Specifically, a number of genes regulated by the mixtures were identified in this study, which were not affected by exposure to any single component. These genes are implicated in multiple biological functions and processes, including fatty acid metabolism and transport, xenobiotic metabolism, immune responses, and oxidative stress. More than 80% of the altered genes in the PFOA- and PFOS-dominant mixture groups were of the same gene set, while the gene expression profiles from single PFOA and PFOS exposures were not as similar. This work contributes to the development of toxicogenomic approaches in combined toxicity assessment and allows for comprehensive insights into the combined action of PFCs mixtures in multiple environmental matrices. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | PERFLUOROOCTANE SULFONIC-ACID ; GENE-EXPRESSION PROFILES ; SURFACE-WATER SAMPLES ; PEROXISOME PROLIFERATORS ; PERFLUOROALKYL ACIDS ; OXIDATIVE STRESS ; RAINBOW-TROUT ; LIVER ; RAT ; PFOA |
| 资助信息 | National Natural Science Foundation of China [20837004, 20777074] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271727000004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7492]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Anim Ecol & Conservat Biol, Inst Zool, Beijing 100101, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Yanhong,Shi, Xiongjie,Zhang, Hongxia,et al. Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis[J]. AQUATIC TOXICOLOGY,2009,95(1):27-36. |
| APA | Wei, Yanhong,Shi, Xiongjie,Zhang, Hongxia,Wang, Jianshe,Zhou, Bingsheng,&Dai, Jiayin.(2009).Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis.AQUATIC TOXICOLOGY,95(1),27-36. |
| MLA | Wei, Yanhong,et al."Combined effects of polyfluorinated and perfluorinated compounds on primary cultured hepatocytes from rare minnow (Gobiocypris rarus) using toxicogenomic analysis".AQUATIC TOXICOLOGY 95.1(2009):27-36. |
入库方式： OAI收割
来源：水生生物研究所
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