Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements
文献类型：期刊论文
| 作者 | Chen, Chun-Hong1,2,3; Sun, Yong-Hua1; Pei, De-Sheng1; Zhu, Zuo-Yan1,2,3 |
| 刊名 | DEVELOPMENTAL DYNAMICS
 |
| 出版日期 | 2009-11-01 |
| 卷号 | 238期号:11页码:2850-2859 |
| ISSN号 | 1058-8388 |
| 关键词 | zebrafish lats phylogenetic analysis spatiotemporal expression covergence extention movement |
| 通讯作者 | Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements. Developmental Dynamics 238:28502859, 2009. (C) 2009 Wiley-Liss, Inc. |
| 英文摘要 | Large tumor suppressor (Lats) is a Ser/Thr kinase, and it presents an important function in tumor suppression. lats was originally identified in Drosophila and recently in mammals. In mammals, it contains two homologues, lats1 and lats2. In the present study, lats1 and lats2 were characterized from zebrafish (Danio rerio), which is the first report of lats in a nonmammalian vertebrate. The primary structure, genomic organization, and phylogenesis of lats from different species were studied, and the results suggest that lats1 is the direct descendant of invertebrate lats, whereas lats2 is formed by genome duplication. In zebrafish, both lats genes are maternally expressed, while they show distinctly different expression profiles during gastrulation. lats1 is almost ubiquitously expressed through development, and lats2 is more prominently expressed in the non-neural ectoderm region of zebrafish gastrula. Most intriguingly, as revealed by cell tracing and gene expression analysis, morpholino-mediated knockdown of either lats1 or lats2 led to obvious defects of cell migration in gastrulation, indicating the functional significance of lats in gastrulation movements. Developmental Dynamics 238:28502859, 2009. (C) 2009 Wiley-Liss, Inc. |
| 学科主题 | Anatomy & Morphology; Developmental Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Anatomy & Morphology ; Developmental Biology |
| 研究领域[WOS] | Anatomy & Morphology ; Developmental Biology |
| 关键词[WOS] | DROSOPHILA TUMOR-SUPPRESSOR ; CELL MOVEMENTS ; PROTEIN-KINASE ; EXTENSION ; GENE ; CONVERGENCE ; WARTS/LATS ; REGULATOR ; POLARITY ; HOMOLOG |
| 资助信息 | State Key Fundamental Research of China [2010CB126306]; National Natural Science Foundation of China [30771100] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271548800012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7502]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Dept Genet, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Ctr Dev Biol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Chun-Hong,Sun, Yong-Hua,Pei, De-Sheng,et al. Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements[J]. DEVELOPMENTAL DYNAMICS,2009,238(11):2850-2859. |
| APA | Chen, Chun-Hong,Sun, Yong-Hua,Pei, De-Sheng,&Zhu, Zuo-Yan.(2009).Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements.DEVELOPMENTAL DYNAMICS,238(11),2850-2859. |
| MLA | Chen, Chun-Hong,et al."Comparative Expression of Zebrafish Lats1 and Lats2 and Their Implication in Gastrulation Movements".DEVELOPMENTAL DYNAMICS 238.11(2009):2850-2859. |
入库方式： OAI收割
来源：水生生物研究所
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