Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations
文献类型：期刊论文
| 作者 | Wang, D.1; Mao, H. -L.2; Chen, H. -X.1; Liu, H. -Q.3,4; Gui, J. -F.1 |
| 刊名 | ANIMAL GENETICS
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 40期号:6页码:978-981 |
| ISSN号 | 0268-9146 |
| 关键词 | AFLP all-male Pelteobagrus fulvidraco X-linked SCAR marker Y-linked SCAR marker YY super-male |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China |
| 中文摘要 | P>Sex controls have been performed in some farmed fish species because of significant growth differences between females and males. In yellow catfish (Pelteobagrus fulvidraco), adult males are three times larger than female adults. In this study, six Y- and X-linked amplified fragment length polymorphism fragments were screened by sex-genotype pool bulked segregant analysis and individual screening. Interestingly, sequence analysis identified two pairs of allelic genes, Pf33 and Pf62. Furthermore, the cloned flanking sequences revealed several Y- and X-specific polymorphisms, and four Y-linked or X-linked sequence characterized amplified region (SCAR) primer pairs were designed and converted into Y- and X-linked SCAR markers. Consequently, these markers were successfully used to identify genetic sex and YY super-males, and applied to all-male population production. Thus, we developed a novel and simple technique to help commercial production of YY super-males and all-male populations in the yellow catfish. |
| 英文摘要 | P>Sex controls have been performed in some farmed fish species because of significant growth differences between females and males. In yellow catfish (Pelteobagrus fulvidraco), adult males are three times larger than female adults. In this study, six Y- and X-linked amplified fragment length polymorphism fragments were screened by sex-genotype pool bulked segregant analysis and individual screening. Interestingly, sequence analysis identified two pairs of allelic genes, Pf33 and Pf62. Furthermore, the cloned flanking sequences revealed several Y- and X-specific polymorphisms, and four Y-linked or X-linked sequence characterized amplified region (SCAR) primer pairs were designed and converted into Y- and X-linked SCAR markers. Consequently, these markers were successfully used to identify genetic sex and YY super-males, and applied to all-male population production. Thus, we developed a novel and simple technique to help commercial production of YY super-males and all-male populations in the yellow catfish. |
| 学科主题 | Agriculture, Dairy & Animal Science; Genetics & Heredity |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Agriculture, Dairy & Animal Science ; Genetics & Heredity |
| 研究领域[WOS] | Agriculture ; Genetics & Heredity |
| 关键词[WOS] | BULKED SEGREGANT ANALYSIS ; FULVIDRACO RICHARDSON ; RAINBOW-TROUT ; SEX ; IDENTIFICATION ; TILAPIA ; FEMALE ; AFLP ; GROWTH ; CARP |
| 资助信息 | National Major Basic Research Program [2004CB117401, 2010CB126301]; President Project of Chinese Academy of Sciences [1072]; Innovation Project of Chinese Academy of Sciences [KSCX2-YW-N-020]; Project of Institute of Hydrobiology, Chinese Academy of Sciences [075A011301]; Open Project of State Key laboratory of Freshwater Ecology and Biotechnology [2009FB01] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000271519700025 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7506]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grad Sch, Wuhan 430072, Peoples R China 2.Nanchang Univ, Sch Life Sci, Nanchang 330031, Peoples R China 3.Minist Water Resources, Inst Hydroecol, Wuhan 430079, Peoples R China 4.Chinese Acad Sci, Wuhan 430079, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, D.,Mao, H. -L.,Chen, H. -X.,et al. Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations[J]. ANIMAL GENETICS,2009,40(6):978-981. |
| APA | Wang, D.,Mao, H. -L.,Chen, H. -X.,Liu, H. -Q.,&Gui, J. -F..(2009).Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations.ANIMAL GENETICS,40(6),978-981. |
| MLA | Wang, D.,et al."Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations".ANIMAL GENETICS 40.6(2009):978-981. |
入库方式： OAI收割
来源：水生生物研究所
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