Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120
文献类型：期刊论文
| 作者 | Dong, Yanling; Xu, Xudong |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2009-11-10 |
| 卷号 | 19期号:11页码:1477-1483 |
| 关键词 | Anabaena Outer membrane Siderophore receptors Iron deficiency |
| ISSN号 | 1002-0071 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Iron deficiency can induce cyanobacteria to synthesize siderophore receptor proteins on the outer membrane to enhance the uptake of iron. In this study, an outer membrane of high purity was prepared from Anabaena sp. PCC 7120 based on aqueous polymer two-phase partitioning and discontinuous sucrose density ultra-centrifugation, and the induction of outer membrane proteins by iron deficiency was investigated using 2-D gel electrophoresis. At least. five outer membrane proteins were newly synthesized or significantly up-regulated in cells transferred to iron-deficient conditions, which were all identified to be siderophore receptor proteins according to MALDI-TOF-MS analyses. Bacterial luciferase reporter genes luxAB were employed to monitor the transcription of the encoding genes. The genes were induced by iron deficiency at the transcriptional level in different responsive modes. Luciferase activity expressed from an iron-regulated promoter may be used as a bioreporter for utilizable iron in natural water samples. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved. |
| 英文摘要 | Iron deficiency can induce cyanobacteria to synthesize siderophore receptor proteins on the outer membrane to enhance the uptake of iron. In this study, an outer membrane of high purity was prepared from Anabaena sp. PCC 7120 based on aqueous polymer two-phase partitioning and discontinuous sucrose density ultra-centrifugation, and the induction of outer membrane proteins by iron deficiency was investigated using 2-D gel electrophoresis. At least. five outer membrane proteins were newly synthesized or significantly up-regulated in cells transferred to iron-deficient conditions, which were all identified to be siderophore receptor proteins according to MALDI-TOF-MS analyses. Bacterial luciferase reporter genes luxAB were employed to monitor the transcription of the encoding genes. The genes were induced by iron deficiency at the transcriptional level in different responsive modes. Luciferase activity expressed from an iron-regulated promoter may be used as a bioreporter for utilizable iron in natural water samples. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | SP STRAIN PCC-7120 ; SYNECHOCYSTIS-SP PCC-6803 ; CYANOBACTERIUM ANABAENA ; OXIDATIVE STRESS ; SIDEROPHORE ; GENES ; SCHIZOKINEN ; EXPRESSION ; TRANSPORT ; NTCA |
| 收录类别 | SCI |
| 资助信息 | State Key Basic Research Development Program [2008CB418001]; National Natural Science Foundation of China [30825003] |
| 语种 | 英语 |
| WOS记录号 | WOS:000271340100003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7508]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, Yanling,Xu, Xudong. Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120[J]. PROGRESS IN NATURAL SCIENCE,2009,19(11):1477-1483. |
| APA | Dong, Yanling,&Xu, Xudong.(2009).Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120.PROGRESS IN NATURAL SCIENCE,19(11),1477-1483. |
| MLA | Dong, Yanling,et al."Outer membrane proteins induced by iron deficiency in Anabaena sp PCC 7120".PROGRESS IN NATURAL SCIENCE 19.11(2009):1477-1483. |
入库方式： OAI收割
来源：水生生物研究所
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