中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Detection of microcystins in environmental samples using surface plasmon resonance biosensor

文献类型:期刊论文

作者Hu, Chenlin1,2; Gan, Nanqin1; Chen, Yuanyuan3; Bi, Lijun3; Zhang, Xianen3; Song, Lirong1
刊名TALANTA
出版日期2009-11-15
卷号80期号:1页码:407-410
ISSN号0039-9140
关键词Microcystins Surface plasmon resonance (SPR) Biosensor ELISA
通讯作者Song, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
中文摘要An indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L-1. Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
英文摘要An indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L(-1). Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
学科主题Chemistry ; Analytical
WOS标题词Science & Technology ; Physical Sciences
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]SMALL MOLECULES ; IMMUNOASSAY ; IMMUNOSENSORS ; LR
资助信息National Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1]
收录类别SCI
语种英语
WOS记录号WOS:000271055700060
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/7530]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Inst Biophys, Analyt Biotechnol State Key Lab Macromol, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714
Hu, Chenlin,Gan, Nanqin,Chen, Yuanyuan,et al. Detection of microcystins in environmental samples using surface plasmon resonance biosensor[J]. TALANTA,2009,80(1):407-410.
APA Hu, Chenlin,Gan, Nanqin,Chen, Yuanyuan,Bi, Lijun,Zhang, Xianen,&Song, Lirong.(2009).Detection of microcystins in environmental samples using surface plasmon resonance biosensor.TALANTA,80(1),407-410.
MLA Hu, Chenlin,et al."Detection of microcystins in environmental samples using surface plasmon resonance biosensor".TALANTA 80.1(2009):407-410.

入库方式: OAI收割

来源:水生生物研究所

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