中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Detection of microcystins in environmental samples using surface plasmon resonance biosensor

文献类型:期刊论文

AuthorHu, Chenlin1,2; Gan, Nanqin1; Chen, Yuanyuan3; Bi, Lijun3; Zhang, Xianen3; Song, Lirong1
SourceTALANTA
Issued Date2009-11-15
Volume80Issue:1Pages:407-410
KeywordMicrocystins Surface plasmon resonance (SPR) Biosensor ELISA
ISSN0039-9140
Corresponding AuthorSong, LR, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Donghu Nanlu 7, Wuhan 430072, Peoples R China
AbstractAn indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L-1. Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
English AbstractAn indirect inhibitive surface plasmon resonance (SPR) immunoassay was developed for the microcystins (MCs) detection. The bioconjugate of MC-LR and bovine serum albumin (BSA) was immobilized on a CM5 sensor chip. A serial premixture of MC-LR standards (or samples) and monoclonal antibody (mAb) were injected over the functional sensor surface, and the subsequent specific immunoreaction was monitored on the BIAcore 3000 biosensor and generated a signal with an increasing intensity in response to the decreasing MCs concentration. The developed SPR immunoassay has a wide quantitative range in 1-100 mu g L(-1). Although not as sensitive as conventional enzyme-linked immunosorbent assay (ELISA), the SPR biosensor offered unique advantages: (I) the sensor chip could be reusable without any significant loss in its binding activity after 50 assay-regeneration cycles, (2) one single assay could be accomplished in 50 min (including 30-min preincubation and 20-min BIAcore analysis), and (3) this method did not require multiple steps. The SPR biosensor was also used to detect MCs in environmental samples, and the results compared well with those obtained by ELISA. We conclude that the SPR biosensor offers outstanding advantages for the MCs detection and may be further developed as a field-portable sensor for real-time monitoring of MCs on site in the near future. (C) 2009 Published by Elsevier B.V.
WOS HeadingsScience & Technology ; Physical Sciences
SubjectChemistry ; Analytical
WOS SubjectChemistry, Analytical
WOS Subject ExtendedChemistry
WOS Keyword PlusSMALL MOLECULES ; IMMUNOASSAY ; IMMUNOSENSORS ; LR
SponsorshipNational Natural Science Foundation of China [20407017]; Chinese Academy of Sciences [KZCX-1-YW-14-1]
Indexed TypeSCI
Language英语
WOS IDWOS:000271055700060
Available Date2010-10-13