Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography
文献类型：期刊论文
| 作者 | Wu, Xingqiang1,2; Xiao, Bangding1; Li, Renhui1; Wang, Zhi1,2; Chen, Xiaoguo3; Chen, Xudong1 |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2009-10-05 |
| 卷号 | 651期号:2页码:241-247 |
| ISSN号 | 0003-2670 |
| 关键词 | Microcystin MMPB (2-methyl-3-methoxy-4-phenylbutyric acid) Cyanobacterial Permanganate-periodate Liquid chromatography-diode array detection |
| 通讯作者 | Xiao, BD, Chinese Acad Sci, Inst Hydrobiol, Donghu S Rd 7, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Microcystins (MCs) comprise a family of more than 80 related cyclic hepatotoxic heptapeptides. Oxidation of MCs causes cleavage of the chemically unique C-20 beta-amino acid (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) amino to form 2-methyl-3-methoxy-4-phenylbutanoic acid (MMPB), which has been exploited to enable analysis of the entire family. In the present study, the reaction conditions (e.g. concentration of the reactants. temperature and pH) used in the production of MMPB by oxidation of cyanobacterial samples with permanganate-periodate were optimized through a series of well-controlled batch experiments. The oxidation product (MMPB) was then directly analyzed by high-performance liquid chromatography with diode array detection. The results of this study provided insight into the influence of reaction conditions on the yield of MMPB. Specifically, the optimal conditions, including a high dose of permanganate (>= 50 mM) in saturated periodate solution at ambient temperature under alkaline conditions (pH similar to 9) over 1-4 h were proposed, as indicated by a MMPB yield of greater than 85%. The technique developed here was applied to determine the total concentration of MCs in cyanobacterial bloom samples, and indicated that the MMPB technique was a highly sensitive and accurate method of quantifying total MCs. Additionally, these results will aid in development of a highly effective analytical method for detection of MMPB as an oxidation product for evaluation of total MCs in a wide range of environmental sample matrices, including natural waters, soils (sediments) and animal tissues. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | Microcystins (MCs) comprise a family of more than 80 related cyclic hepatotoxic heptapeptides. Oxidation of MCs causes cleavage of the chemically unique C(20) beta-amino acid (2S, 3S, 8S, 9S)-3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid (Adda) amino to form 2-methyl-3-methoxy-4-phenylbutanoic acid (MMPB), which has been exploited to enable analysis of the entire family. In the present study, the reaction conditions (e.g. concentration of the reactants. temperature and pH) used in the production of MMPB by oxidation of cyanobacterial samples with permanganate-periodate were optimized through a series of well-controlled batch experiments. The oxidation product (MMPB) was then directly analyzed by high-performance liquid chromatography with diode array detection. The results of this study provided insight into the influence of reaction conditions on the yield of MMPB. Specifically, the optimal conditions, including a high dose of permanganate (>= 50 mM) in saturated periodate solution at ambient temperature under alkaline conditions (pH similar to 9) over 1-4 h were proposed, as indicated by a MMPB yield of greater than 85%. The technique developed here was applied to determine the total concentration of MCs in cyanobacterial bloom samples, and indicated that the MMPB technique was a highly sensitive and accurate method of quantifying total MCs. Additionally, these results will aid in development of a highly effective analytical method for detection of MMPB as an oxidation product for evaluation of total MCs in a wide range of environmental sample matrices, including natural waters, soils (sediments) and animal tissues. (C) 2009 Elsevier B.V. All rights reserved. |
| 学科主题 | Chemistry, Analytical |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | PROTEIN PHOSPHATASE INHIBITION ; GREEN-ALGAE CYANOBACTERIA ; PEPTIDE TOXINS ; WATER ; HEPATOTOXINS ; BIOACCUMULATION ; EXTRACTION ; AERUGINOSA ; TOXICITY ; EXPOSURE |
| 资助信息 | National Key Project for Basic Research [2008CB418101]; Chinese Academy of Sciences (CAS) [KZCX2-YW-426]; National High Technology Research and Development Program of China [2007AA06Z304] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270745600016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7560]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wuhan Univ Technol, Wuhan 430070, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Xingqiang,Xiao, Bangding,Li, Renhui,et al. Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography[J]. ANALYTICA CHIMICA ACTA,2009,651(2):241-247. |
| APA | Wu, Xingqiang,Xiao, Bangding,Li, Renhui,Wang, Zhi,Chen, Xiaoguo,&Chen, Xudong.(2009).Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography.ANALYTICA CHIMICA ACTA,651(2),241-247. |
| MLA | Wu, Xingqiang,et al."Rapid quantification of total microcystins in cyanobacterial samples by periodate-permanganate oxidation and reversed-phase liquid chromatography".ANALYTICA CHIMICA ACTA 651.2(2009):241-247. |
入库方式： OAI收割
来源：水生生物研究所
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