Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)
文献类型：期刊论文
| 作者 | Xu, Z.1,2; Chang, M. X.1; Qi, Z. T.1; Huang, B.1; Nie, P.1 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2009-10-15 |
| 卷号 | 131期号:3-4页码:268-272 |
| 关键词 | Joining (J) chain CDNA sequence Expression Chinese soft-shelled turtle Pelodiscus sinensis Bacterial infection |
| ISSN号 | 0165-2427 |
| 通讯作者 | Nie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | The immunoglobulin (Ig) joining (J) chain plays an important role in the formation of polymeric Igs and their transport into secretions. In the present study, the cDNA sequence of J chain has been cloned from the Chinese soft-shelled turtle (Pelodiscus sinensis) by reverse transcription (RT)-PCR and rapid amplification of cDNA ends (RACE). The cDNA sequence is 2347 bp in length and contains an open reading frame of 480 bp encoding 160 aa including the signal sequence. The deduced amino acid sequence has a high degree of homology with that of an already reported turtle J chain (80.7%), and of chicken (71.3%). By using real-time quantitative RT-PCR analysis, a significant up-regulation of J-chain transcripts was observed in spleen, kidney and blood of turtles injected with inactivated Aeromonas hydrophila, indicating the immune role of J chain in response to bacterial infection. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | (J)-CHAIN CDNA ; CLONING ; SEQUENCE ; GENE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30571412]; National Natural Science Foundation of China (NSFC) [U0631010] |
| 语种 | 英语 |
| WOS记录号 | WOS:000270487400013 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7562]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Z.,Chang, M. X.,Qi, Z. T.,et al. Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,131(3-4):268-272. |
| APA | Xu, Z.,Chang, M. X.,Qi, Z. T.,Huang, B.,&Nie, P..(2009).Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,131(3-4),268-272. |
| MLA | Xu, Z.,et al."Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 131.3-4(2009):268-272. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。