Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter
文献类型：期刊论文
| 作者 | Jiang, Jun; Zhang, Yi-Bing; Li, Shun; Yu, Fei-Fei; Sun, Fan; Gui, Jian-Fang |
| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 46期号:15页码:3131-3140 |
| 关键词 | Gig2 Interferon Interferon-stimulated gene (ISG) IRF7 Poly I:C Promoter Interferon-stimulated response elements Expression regulation |
| ISSN号 | 0161-5890 |
| 通讯作者 | Zhang, YB, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, 7 Donghu S Rd, Wuhan 430072, Peoples R China |
| 中文摘要 | Grass carp hemorrhagic virus (GCHV)-induced gene 2 (Gig2) is a novel gene previously identified from UV-inactivated GCHV-treated Carassius auratus blastulae embryonic (CAB) cells, suggesting that it should play a pivotal role in the interferon (IFN) antiviral response. In this study, a polyclonal anti-Gig2 antiserum was generated and used to study the inductive expression pattern by Western blot analysis, showing no basal expression in normal CAB cells but a significant up-regulation upon UV-inactivated GCHV, polyinosinic:polycytidylic acid (Poly I:Q and recombinant IFN (rIFN). However, constitutive expression of Gig2 is observed in all tested tissues from grass carp (Ctenopharyngodon idellus), and Poly I:C injection increases the relative amount of Gig2 protein in skin, spleen, trunk kidney, gill, hindgut and thymus. Moreover, the genomic sequence covering the whole Gig2 ORF and the upstream promoter region were amplified by genomic walking. Significantly, the Gig2 promoter contains three IFN-stimulated response elements (ISREs), nine GAAA/TfTC motifs and five gamma-IFN activating sites (GAS), which are the characteristics of genes responsive to both type I IFN and type 11 IFN. Subsequently, the complete Gig2 promoter sequence was cloned into pGL3-Basic vector, and its activity was measured by luciferase assays in the transfected CAB cells. The Gig2 promoter-driven construct is highly induced in CAB cells after treatment with Poly I:C or rIFN, and the functional capability is dependent on IFN regulatory factor 7 (IRF7), because its activity can be stimulated by IRF7. Collectively, the data provide strong evidence that Gig2 is indeed a novel IFN inducible gene and its expression is likely dependent on IRF7 upon Poly I:C or IFN. (C) 2009 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Grass carp hemorrhagic virus (GCHV)-induced gene 2 (Gig2) is a novel gene previously identified from UV-inactivated GCHV-treated Carassius auratus blastulae embryonic (CAB) cells, suggesting that it should play a pivotal role in the interferon (IFN) antiviral response. In this study, a polyclonal anti-Gig2 antiserum was generated and used to study the inductive expression pattern by Western blot analysis, showing no basal expression in normal CAB cells but a significant up-regulation upon UV-inactivated GCHV, polyinosinic:polycytidylic acid (Poly I:Q and recombinant IFN (rIFN). However, constitutive expression of Gig2 is observed in all tested tissues from grass carp (Ctenopharyngodon idellus), and Poly I:C injection increases the relative amount of Gig2 protein in skin, spleen, trunk kidney, gill, hindgut and thymus. Moreover, the genomic sequence covering the whole Gig2 ORF and the upstream promoter region were amplified by genomic walking. Significantly, the Gig2 promoter contains three IFN-stimulated response elements (ISREs), nine GAAA/TfTC motifs and five gamma-IFN activating sites (GAS), which are the characteristics of genes responsive to both type I IFN and type 11 IFN. Subsequently, the complete Gig2 promoter sequence was cloned into pGL3-Basic vector, and its activity was measured by luciferase assays in the transfected CAB cells. The Gig2 promoter-driven construct is highly induced in CAB cells after treatment with Poly I:C or rIFN, and the functional capability is dependent on IFN regulatory factor 7 (IRF7), because its activity can be stimulated by IRF7. Collectively, the data provide strong evidence that Gig2 is indeed a novel IFN inducible gene and its expression is likely dependent on IRF7 upon Poly I:C or IFN. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Immunology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; NF-KAPPA-B ; CARP-HEMORRHAGE-VIRUS ; RAINBOW-TROUT ; ATLANTIC SALMON ; PARALICHTHYS-OLIVACEUS ; CAB CELLS ; MX GENE ; MOLECULAR CHARACTERIZATION ; JAPANESE FLOUNDER |
| 收录类别 | SCI |
| 资助信息 | 973 National Basic Research Program of China [2004CB117403]; 863 High Technology Research Program of China [2007AA09Z423]; National Natural Science Foundation of China [30671617, 30871922]; Innovation Project of Institute of Hydrobiology, Chinese Academy of Sciences [085A01-1-301] |
| 语种 | 英语 |
| WOS记录号 | WOS:000270489800029 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7564]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Jun,Zhang, Yi-Bing,Li, Shun,et al. Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter[J]. MOLECULAR IMMUNOLOGY,2009,46(15):3131-3140. |
| APA | Jiang, Jun,Zhang, Yi-Bing,Li, Shun,Yu, Fei-Fei,Sun, Fan,&Gui, Jian-Fang.(2009).Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter.MOLECULAR IMMUNOLOGY,46(15),3131-3140. |
| MLA | Jiang, Jun,et al."Expression regulation and functional characterization of a novel interferon inducible gene Gig2 and its promoter".MOLECULAR IMMUNOLOGY 46.15(2009):3131-3140. |
入库方式： OAI收割
来源：水生生物研究所
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