Extraction of genomic DNA using a new amino silica monolithic column
文献类型：期刊论文
| 作者 | Liu, Lijia1; Yu, Shengbing1; Yang, Shuixian1; Zhou, Ping1; Hu, Jiming1; Zhang, Yibing2 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 32期号:15-16页码:2752-2758 |
| 关键词 | DNA purification Genomic DNA Monolithic column Solid-phase extraction |
| ISSN号 | 1615-9306 |
| 通讯作者 | Zhou, P, Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 中文摘要 | A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(beta-aminoethyl)-gamma-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 +/- 5.2% (X +/- RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 +/- 5.6% (X +/- SD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale. |
| 英文摘要 | A new amino silica monolithic column was developed for DNA extraction in a miniaturized format. The monolithic column was prepared in situ by polymerization of tetraethoxysilane (TEOS) and N-(beta-aminoethyl)-gamma-aminopropylmethyldimethoxysilane (AEAPMDMS). DNA was loaded in 50 mM tris(hydroxylmethyl)aminomethane-EDTA buffer at pH 7.0 and eluted with 300 mM potassium phosphate solution at pH 10.0. Under optimal condition, a 6.0-cm monolithic column provided a capacity of 56 ng DNA with an extraction efficiency of 71 +/- 5.2% (X +/- RSD). When the amino silica monolithic column was applied to extract genomic DNA from the whole blood of crucian carp, an extraction efficiency of 52 +/- 5.6% (X +/- SD) was obtained by three extractions. Since the chaotropic-based sample loading and organic solvent wash steps were avoided in this procedure, the purified DNA was suitable for downstream processes such as PCR. This amino silica monolithic column was demonstrated to allow rapid and efficient DNA purification in microscale. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 学科主题 | Chemistry ; Analytical |
| 类目[WOS] | Chemistry, Analytical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | BACTERIAL MAGNETIC PARTICLES ; SOLID-PHASE EXTRACTION ; NUCLEIC-ACIDS ; MICROFLUIDIC CHIPS ; PLASMID DNA ; PURIFICATION ; MICROCHIP ; SAMPLES ; SYSTEM ; FABRICATION |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of China [20875073] |
| 语种 | 英语 |
| WOS记录号 | WOS:000269413400032 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7598]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lijia,Yu, Shengbing,Yang, Shuixian,et al. Extraction of genomic DNA using a new amino silica monolithic column[J]. JOURNAL OF SEPARATION SCIENCE,2009,32(15-16):2752-2758. |
| APA | Liu, Lijia,Yu, Shengbing,Yang, Shuixian,Zhou, Ping,Hu, Jiming,&Zhang, Yibing.(2009).Extraction of genomic DNA using a new amino silica monolithic column.JOURNAL OF SEPARATION SCIENCE,32(15-16),2752-2758. |
| MLA | Liu, Lijia,et al."Extraction of genomic DNA using a new amino silica monolithic column".JOURNAL OF SEPARATION SCIENCE 32.15-16(2009):2752-2758. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。