Benzolactam (BL) enhances sAPP secretion in fibroblasts and in PC12 cells
文献类型：期刊论文
| 作者 | Dolores lbarreta ; Miriam Duchen ; Ma DW(马大为) ; Qiao LX(乔立新) ; Alan P. Kozikowski ; Rene Etcheberrigaray |
| 刊名 | NeuroReport
 |
| 出版日期 | 1999 |
| 卷号 | 10页码:1035-1040 |
| ISSN号 | 0959-4965 |
| 其他题名 | 苯并内酰胺增加 SAPP 在两种细胞中的分泌作用 |
| 通讯作者 | Rene Etcheberrigaray |
| 英文摘要 | Activation of protein kinase C is known to favor the α-secretase processing of the Alzheimer's disease (AD) amyloid precursor protein (APP), resulting in the generation of non-amyloidogenic soluble APP (sAPP0. Consequentyl, the relative secretion of amyloidogenic Aβ=1-40 and Aβ=1-42(3) is reduced. This is particularly relevant since fibroblasts and other cells ecpressing APP and presenilin AD mutations secrete increased amounts of total Aβ and/or increased ratios of Aβ=1-42(3)/Aβ=1-40. Interstingly, PKC defects have been found in AD brain (α and β isoforms) and in fibroblasts (α isoform) from AD patients. Here, we use a novel PKC activator (benzolactan, BL) with improced selectivity for the α，β and γ isoforms to enhance sAPP secretion if fibroblasts from AD patients and in PC12 cells. Incubation (2h) of AD fibroblasts with BL (1 and 10M) resulted in significant increases of sAPP secretion over basal level. sAPP secretion in BL-treated AD cells was also slightly higher compared to control BL--treated fibroblasts, which only showed significant increases of sAPP secretion after treatment with 10μM BL. Staurosporine (aPKC inhibitor) eliminated the effects of BL in both control and AD fibroblasts. BL and a related compound (LQ12) also caused and ~3-fold sAPP secretion in PC12PKC activator may prove useful to favor non-amyloidogenic APP processing and is,therefore, of potential therapectic alue. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000079904900028 |
| 公开日期 | 2013-02-19 |
| 源URL | [http://202.127.28.38/handle/331003/15345]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Dolores lbarreta,Miriam Duchen,Ma DW,et al. Benzolactam (BL) enhances sAPP secretion in fibroblasts and in PC12 cells[J]. NeuroReport,1999,10:1035-1040. |
| APA | Dolores lbarreta,Miriam Duchen,马大为,乔立新,Alan P. Kozikowski,&Rene Etcheberrigaray.(1999).Benzolactam (BL) enhances sAPP secretion in fibroblasts and in PC12 cells.NeuroReport,10,1035-1040. |
| MLA | Dolores lbarreta,et al."Benzolactam (BL) enhances sAPP secretion in fibroblasts and in PC12 cells".NeuroReport 10(1999):1035-1040. |
入库方式： OAI收割
来源：上海有机化学研究所
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