Transcriptome of embryonic and neonatal mouse cortex by high-throughput RNA sequencing
文献类型：期刊论文
| 作者 | Han, Xinwei1,3,4; Wu, Xia1,3; Chung, Wen-Yu3,5; Li, Tao1,3,9; Nekrutenko, Anton2,3,4; Altman, Naomi S.3,6; Chen, Gong1,3,4; Ma, Hong1,4,5,7,8 |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
 |
| 出版日期 | 2009-08-04 |
| 卷号 | 106期号:31页码:12741-12746 |
| 关键词 | E18 P7 brain transcription factors neural diseases |
| ISSN号 | 0027-8424 |
| 通讯作者 | Chen, G, Penn State Univ, Dept Biol, 201 Life Sci Bldg, University Pk, PA 16802 USA |
| 中文摘要 | Brain structure and function experience dramatic changes from embryonic to postnatal development. Microarray analyses have detected differential gene expression at different stages and in disease models, but gene expression information during early brain development is limited. We have generated >27 million reads to identify mRNAs from the mouse cortex for>16,000 genes at either embryonic day 18 (E18) or postnatal day 7 (P7), a period of significant synapto-genesis for neural circuit formation. In addition, we devised strategies to detect alternative splice forms and uncovered more splice variants. We observed differential expression of 3,758 genes between the 2 stages, many with known functions or predicted to be important for neural development. Neurogenesis-related genes, such as those encoding Sox4, Sox11, and zinc-finger proteins, were more highly expressed at E18 than at P7. In contrast, the genes encoding synaptic proteins such as synaptotagmin, complexin 2, and syntaxin were up-regulated from E18 to P7. We also found that several neurological disorder-related genes were highly expressed at E18. Our transcriptome analysis may serve as a blueprint for gene expression pattern and provide functional clues of previously unknown genes and disease-related genes during early brain development. |
| 英文摘要 | Brain structure and function experience dramatic changes from embryonic to postnatal development. Microarray analyses have detected differential gene expression at different stages and in disease models, but gene expression information during early brain development is limited. We have generated >27 million reads to identify mRNAs from the mouse cortex for>16,000 genes at either embryonic day 18 (E18) or postnatal day 7 (P7), a period of significant synapto-genesis for neural circuit formation. In addition, we devised strategies to detect alternative splice forms and uncovered more splice variants. We observed differential expression of 3,758 genes between the 2 stages, many with known functions or predicted to be important for neural development. Neurogenesis-related genes, such as those encoding Sox4, Sox11, and zinc-finger proteins, were more highly expressed at E18 than at P7. In contrast, the genes encoding synaptic proteins such as synaptotagmin, complexin 2, and syntaxin were up-regulated from E18 to P7. We also found that several neurological disorder-related genes were highly expressed at E18. Our transcriptome analysis may serve as a blueprint for gene expression pattern and provide functional clues of previously unknown genes and disease-related genes during early brain development. |
| WOS标题词 | Science & Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Multidisciplinary Sciences |
| 研究领域[WOS] | Science & Technology - Other Topics |
| 关键词[WOS] | GENE-EXPRESSION ; HUMAN GENOME ; DNA MICROARRAY ; BRAIN ; SYNAPTOGENESIS ; IDENTIFICATION ; AUTOPHAGY ; NEURONS ; ARRAYS ; MICE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268667600034 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7626]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Penn State Univ, Dept Biol, University Pk, PA 16802 USA 2.Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA 3.Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA 4.Penn State Univ, Intercoll Grad Program Genet, University Pk, PA 16802 USA 5.Penn State Univ, Dept Comp Sci & Engn, University Pk, PA 16802 USA 6.Penn State Univ, Dept Stat, University Pk, PA 16802 USA 7.Fudan Univ, State Key Lab, Genet Engn & Inst Plant Biol, Ctr Evolutionary Biol,Sch Life Sci, Shanghai 200433, Peoples R China 8.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China 9.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Xinwei,Wu, Xia,Chung, Wen-Yu,et al. Transcriptome of embryonic and neonatal mouse cortex by high-throughput RNA sequencing[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2009,106(31):12741-12746. |
| APA | Han, Xinwei.,Wu, Xia.,Chung, Wen-Yu.,Li, Tao.,Nekrutenko, Anton.,...&Ma, Hong.(2009).Transcriptome of embryonic and neonatal mouse cortex by high-throughput RNA sequencing.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,106(31),12741-12746. |
| MLA | Han, Xinwei,et al."Transcriptome of embryonic and neonatal mouse cortex by high-throughput RNA sequencing".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106.31(2009):12741-12746. |
入库方式： OAI收割
来源：水生生物研究所
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