Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis
文献类型：期刊论文
| 作者 | Li, Chuang-Ju1,3; Wei, Qi-Wei1,3; Zhou, Li2; Cao, Hong4; Zhang, Yan1,3; Gui, Jian-Fang2 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
 |
| 出版日期 | 2009-09-01 |
| 卷号 | 154期号:1页码:127-134 |
| ISSN号 | 1095-6433 |
| 关键词 | Chinese sturgeon Evolution Gene expression Somatostatin |
| 通讯作者 | Wei, QW, Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou 434000, Peoples R China |
| 中文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS14 sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS14 at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development. (C) 2009 Elsevier Inc. All rights reserved. |
| 英文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry. SMART cDNA was synthesized from the hypothalamus of Chinese sturgeon, and the full-length cDNAs of two somatostatin (SS) genes were cloned and sequenced. The first cDNA (AsSS1) encodes a 116-amino acid protein that contains the SS(14) sequence at its C-terminal extremity. AsSS1 shows high identity to that of human and other vertebrates. The second cDNA (AsSS2) encodes a 111-amino acid protein that contains the somatostatin variant [Pro(2)]-SS(14) at its C-terminal extremity. Both the two SS mRNAs were expressed in brain and pituitary with different mRNA levels. But in peripheral tissues, AsSS2 was more widely distributed than AsSS1. High mRNA levels of AsSS2 were found in liver, kidney and heart, while low mRNA levels of AsSS2 were also detected in ovary. Throughout embryogenesis and early larval development only AsSS2 mRNAs were detected. Furthermore, in the hypothalamus of one to five year-old Chinese sturgeon, AsSS2 but not AsSS1 maintained stable expression. The mRNA distribution suggests that the Chinese sturgeon AsSS2 products play important physiological functions in adult fish as well as in cell growth and organ differentiation in embryo and larva development. (C) 2009 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Physiology; Zoology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Physiology ; Zoology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Physiology ; Zoology |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; MESSENGER-RNAS ; RAINBOW-TROUT ; GROWTH-HORMONE ; DIFFERENTIAL EXPRESSION ; EMBRYONIC-DEVELOPMENT ; POLYGENIC EXPRESSION ; ENDOCRINE PANCREAS ; UROTENSIN-II ; CLONING |
| 资助信息 | Yangtze River Fisheries Research institute ; Chinese Academy of Fisheries and the Open project of State Key Laboratory of Freshwater Ecology and Biotechnology [20081713007] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000268625600016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7638]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Fisheries Sci, Yangtze River Fisheries Res Inst, Minist Agr China, Key Lab Freshwater Biodivers Conservat & Utilizat, Jinzhou 434000, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Chinese Acad Fisheries Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China 4.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Chuang-Ju,Wei, Qi-Wei,Zhou, Li,et al. Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2009,154(1):127-134. |
| APA | Li, Chuang-Ju,Wei, Qi-Wei,Zhou, Li,Cao, Hong,Zhang, Yan,&Gui, Jian-Fang.(2009).Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,154(1),127-134. |
| MLA | Li, Chuang-Ju,et al."Molecular and expression characterization of two somatostatin genes in the Chinese sturgeon, Acipenser sinensis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 154.1(2009):127-134. |
入库方式： OAI收割
来源：水生生物研究所
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