Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to Perfluorooctane Sulfonate
文献类型：期刊论文
| 作者 | Shi, Xiongjie1; Yeung, Leo W. Y.2; Lam, Paul K. S.2; Wu, Rudolf S. S.2; Zhou, Bingsheng1 |
| 刊名 | TOXICOLOGICAL SCIENCES
 |
| 出版日期 | 2009-08-01 |
| 卷号 | 110期号:2页码:334-340 |
| 关键词 | PFOS proteomics embryo mechanism zebrafish |
| ISSN号 | 1096-6080 |
| 通讯作者 | Zhou, BS, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Perfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and in wildlife, and it has the potential for developmental toxicity. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the proteins that are differentially expressed in zebrafish embryos exposed to 0.5 mg/l PFOS until 192 h postfertilization. Two-dimensional electrophoresis coupled with mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that 69 proteins showed altered expression in the treatment group compared to the control group with either increase or decrease in expression levels (more than twofold difference). Of the 69 spots corresponding to the proteins with altered expression, 38 were selected and subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (TOF/TOF) analysis; 18 proteins were identified in this analysis. These proteins can be categorized into diverse functional classes such as detoxification, energy metabolism, lipid transport/steroid metabolic process, cell structure, signal transduction, and apoptosis. Overall, proteomic analysis using zebrafish embryos serves as an in vivo model in environmental risk assessment and provides insight into the molecular events in PFOS-induced developmental toxicity. |
| 英文摘要 | Perfluorooctane sulfonate (PFOS) is widely distributed and persistent in the environment and in wildlife, and it has the potential for developmental toxicity. However, the molecular mechanisms that lead to these toxic effects are not well known. In the present study, proteomic analysis has been performed to investigate the proteins that are differentially expressed in zebrafish embryos exposed to 0.5 mg/l PFOS until 192 h postfertilization. Two-dimensional electrophoresis coupled with mass spectrometry was employed to detect and identify the protein profiles. The analysis revealed that 69 proteins showed altered expression in the treatment group compared to the control group with either increase or decrease in expression levels (more than twofold difference). Of the 69 spots corresponding to the proteins with altered expression, 38 were selected and subjected to matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (TOF/TOF) analysis; 18 proteins were identified in this analysis. These proteins can be categorized into diverse functional classes such as detoxification, energy metabolism, lipid transport/steroid metabolic process, cell structure, signal transduction, and apoptosis. Overall, proteomic analysis using zebrafish embryos serves as an in vivo model in environmental risk assessment and provides insight into the molecular events in PFOS-induced developmental toxicity. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Toxicology |
| 类目[WOS] | Toxicology |
| 研究领域[WOS] | Toxicology |
| 关键词[WOS] | LIFE-CYCLE TEST ; PERFLUORINATED COMPOUNDS ; DEVELOPMENTAL TOXICITY ; PROTEOMIC ANALYSIS ; OXIDATIVE STRESS ; CELL-LINES ; EXPRESSION ; LIVER ; PFOS ; ACID |
| 收录类别 | SCI |
| 资助信息 | National Nature Science Foundation of China [20890113, 20877094]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FBZ10] |
| 语种 | 英语 |
| WOS记录号 | WOS:000267889700008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7652]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Xiongjie,Yeung, Leo W. Y.,Lam, Paul K. S.,et al. Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to Perfluorooctane Sulfonate[J]. TOXICOLOGICAL SCIENCES,2009,110(2):334-340. |
| APA | Shi, Xiongjie,Yeung, Leo W. Y.,Lam, Paul K. S.,Wu, Rudolf S. S.,&Zhou, Bingsheng.(2009).Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to Perfluorooctane Sulfonate.TOXICOLOGICAL SCIENCES,110(2),334-340. |
| MLA | Shi, Xiongjie,et al."Protein Profiles in Zebrafish (Danio rerio) Embryos Exposed to Perfluorooctane Sulfonate".TOXICOLOGICAL SCIENCES 110.2(2009):334-340. |
入库方式： OAI收割
来源：水生生物研究所
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