Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper
文献类型：期刊论文
| 作者 | Huang, Wei; Zhou, Li; Li, Zhi; Gui, Jian-Fang |
| 刊名 | MOLECULAR AND CELLULAR ENDOCRINOLOGY
 |
| 出版日期 | 2009-08-13 |
| 卷号 | 307期号:1-2页码:224-236 |
| 关键词 | Immunofluorescene localization Promoter activity Cyp19a1a/b Grouper Hermaphrodite Sex reversal |
| ISSN号 | 0303-7207 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Aromatase plays a key role in sex differentiation of gonads. In this study, we cloned the full-length cDNA of ovarian aromatase from protogynous hermaphrodite red-spotted grouper (Epinephelus akaara), and prepared the corresponding anti-EaCyp19a1a antiserum. Western blot and immunofluorescence studies revealed ovary-specific expression pattern of EaCyp19a1a in adults and its dynamic expression change during artificial sex reversal. EaCyp19a1a was expressed by follicular cells of follicular layer around oocytes because strong EaCyp19a1a immunofluorescence was observed in the cells of ovaries. During artificial sex reversal, EaCyp19a1a expression dropped significantly from female to male, and almost no any positive EaCyp19a1a signal was observed in testicular tissues. Then, we cloned and sequenced a total of 1967 bp T-flanking sequence of EaCyp19a1a promoter, and showed a number of potential binding sites for some transcriptional factors, such as SOX5, GATA gene family, CREB, AP1, FOXL1, C/EBP, ARE and SF-1. Moreover, we prepared a series of 5' deletion promoter constructs and performed in vitro luciferase assays of EaCyp19a1a promoter activities. The data indicated that the CREB regulation region from -1010 to -898 might be a major cis-acting element to EaCyp19a1a promoter, whereas the elements GATA and SOX5 in the region from -1216 to -1010 might be suppression elements. Significantly, we found a common conserved sequence region in the fish ovary-type aromatase promoters with identities from 93% to 34%. And, the motifs of TATA box, SF-1, SOX5, and CREB existed in the region and were conserved among the most of fish species. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | Aromatase plays a key role in sex differentiation of gonads. In this study, we cloned the full-length cDNA of ovarian aromatase from protogynous hermaphrodite red-spotted grouper (Epinephelus akaara), and prepared the corresponding anti-EaCyp19a1a antiserum. Western blot and immunofluorescence studies revealed ovary-specific expression pattern of EaCyp19a1a in adults and its dynamic expression change during artificial sex reversal. EaCyp19a1a was expressed by follicular cells of follicular layer around oocytes because strong EaCyp19a1a immunofluorescence was observed in the cells of ovaries. During artificial sex reversal, EaCyp19a1a expression dropped significantly from female to male, and almost no any positive EaCyp19a1a signal was observed in testicular tissues. Then, we cloned and sequenced a total of 1967 bp T-flanking sequence of EaCyp19a1a promoter, and showed a number of potential binding sites for some transcriptional factors, such as SOX5, GATA gene family, CREB, AP1, FOXL1, C/EBP, ARE and SF-1. Moreover, we prepared a series of 5' deletion promoter constructs and performed in vitro luciferase assays of EaCyp19a1a promoter activities. The data indicated that the CREB regulation region from -1010 to -898 might be a major cis-acting element to EaCyp19a1a promoter, whereas the elements GATA and SOX5 in the region from -1216 to -1010 might be suppression elements. Significantly, we found a common conserved sequence region in the fish ovary-type aromatase promoters with identities from 93% to 34%. And, the motifs of TATA box, SF-1, SOX5, and CREB existed in the region and were conserved among the most of fish species. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Cell Biology; Endocrinology & Metabolism |
| 类目[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | TILAPIA OREOCHROMIS-NILOTICUS ; MESSENGER-RNA EXPRESSION ; TROUT ONCORHYNCHUS-MYKISS ; BASS DICENTRARCHUS-LABRAX ; EUROPEAN SEA BASS ; P450 AROMATASE ; EPINEPHELUS-COIOIDES ; NILE TILAPIA ; DEVELOPMENTAL EXPRESSION ; CYTOCHROME-P450 AROMATASES |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research [2004CB117401]; National Natural Science Foundation of China ; Guangdong Province [U0631007]; Chinese Academy of Sciences [KSCX2-YW-N-020, 075A011301]; Innovation Project of Institute of Hydrobiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000267837400029 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7654]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wei,Zhou, Li,Li, Zhi,et al. Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2009,307(1-2):224-236. |
| APA | Huang, Wei,Zhou, Li,Li, Zhi,&Gui, Jian-Fang.(2009).Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper.MOLECULAR AND CELLULAR ENDOCRINOLOGY,307(1-2),224-236. |
| MLA | Huang, Wei,et al."Expression pattern, cellular localization and promoter activity analysis of ovarian aromatase (Cyp19a1a) in protogynous hermaphrodite red-spotted grouper".MOLECULAR AND CELLULAR ENDOCRINOLOGY 307.1-2(2009):224-236. |
入库方式： OAI收割
来源：水生生物研究所
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