Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)
文献类型：期刊论文
| 作者 | Chen, Guo-Yuan1,2; Qiu, Shan-Lian1,2; Zhou, Yi-Yong1 |
| 刊名 | RESEARCH IN MICROBIOLOGY
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 160期号:3页码:173-178 |
| ISSN号 | 0923-2508 |
| 关键词 | Ammonia-oxidizing bacteria Ammonia monooxygenase gene Restriction fragment length polymorphism Shallow lakes |
| 通讯作者 | Zhou, YY, Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, 7 Donghu Nan Rd, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH4+) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved. |
| 英文摘要 | Classical cultivation and molecular methods based on the ammonia monooxygenase gene (amoA) were used to study the abundance and diversity of beta-proteobacterial ammonia-oxidizing bacteria (AOB) in lake sediments. The eutrophic and oligotrophic basins of a Chinese shallow lake (Lake Donghu), in terms of ammonium (NH(4)(+)) concentrations, were sampled. The AOB number was significantly lower in the oligotrophic basin, but significantly higher in the eutrophic basin. In addition, using restriction fragment length polymorphism targeting the amoA, ten restriction patterns including six unique ones were found in the eutrophic basin, while five patterns were observed in the oligotrophic basin with only one unique restriction group. Phylogenetic analysis for AOB revealed that Nitrosomonas oligotropha- and Nitrosomonas ureae-related AOB and Nitrosospira-affiliated AOB were ubiquitous; the former dominated in the eutrophic basin (87.2%), while the latter dominated in the oligotrophic basin (65.5%). Furthermore, Nitrosomonas communis-related AOB was only detected in the eutrophic basin, at a small proportion (3.2%). These results indicate significant selection and adaptation of sediment AOB in lakes with differing trophic status. (C) 2009 Elsevier Masson SAS. All rights reserved. |
| 学科主题 | Microbiology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | 16S RIBOSOMAL-RNA ; FRESH-WATER LAKE ; IN-SITU HYBRIDIZATION ; NITRIFYING BACTERIA ; MOLECULAR DIVERSITY ; AMOA ; POPULATIONS ; OXIDATION ; GENE ; NITRIFICATION |
| 资助信息 | National Basic Research Program of China [2008CB418005]; Chinese Academy of Sciences [KZCX2-YW-426-01, KZCX1-YW-14-1] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267685500001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7668]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Lab Water Environm Biochem, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Guo-Yuan,Qiu, Shan-Lian,Zhou, Yi-Yong. Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)[J]. RESEARCH IN MICROBIOLOGY,2009,160(3):173-178. |
| APA | Chen, Guo-Yuan,Qiu, Shan-Lian,&Zhou, Yi-Yong.(2009).Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu).RESEARCH IN MICROBIOLOGY,160(3),173-178. |
| MLA | Chen, Guo-Yuan,et al."Diversity and abundance of ammonia-oxidizing bacteria in eutrophic and oligotrophic basins of a shallow Chinese lake (Lake Donghu)".RESEARCH IN MICROBIOLOGY 160.3(2009):173-178. |
入库方式： OAI收割
来源：水生生物研究所
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