Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus
文献类型：期刊论文
| 作者 | Su, Jianguo1; Yang, Chunrong1; Xiong, Feng2; Wang, Yaping2; Zhu, Zuoyan2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 27期号:1页码:33-39 |
| 关键词 | Gobiocypris rarus Grass carp reovirus Aeromonas hydrophila Toll-like receptor 4 Gene cloning Characterization |
| ISSN号 | 1050-4648 |
| 通讯作者 | Su, JG, NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, 22 Xinong Rd, Yangling 712100, Peoples R China |
| 中文摘要 | Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | RESPIRATORY SYNCYTIAL VIRUS ; MESSENGER-RNA DEGRADATION ; INNATE IMMUNITY ; EXPRESSION ANALYSIS ; TLR4 POLYMORPHISMS ; ADAPTIVE IMMUNITY ; GENE FAMILY ; ENDOTOXIN ; SEQUENCE ; TIR |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30871917]; SRF [14110104] |
| 语种 | 英语 |
| WOS记录号 | WOS:000267478200005 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7674]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.NW A&F Univ, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Jianguo,Yang, Chunrong,Xiong, Feng,et al. Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus[J]. FISH & SHELLFISH IMMUNOLOGY,2009,27(1):33-39. |
| APA | Su, Jianguo,Yang, Chunrong,Xiong, Feng,Wang, Yaping,&Zhu, Zuoyan.(2009).Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus.FISH & SHELLFISH IMMUNOLOGY,27(1),33-39. |
| MLA | Su, Jianguo,et al."Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus".FISH & SHELLFISH IMMUNOLOGY 27.1(2009):33-39. |
入库方式： OAI收割
来源：水生生物研究所
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